FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC134CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC134CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14505
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGTATATAGTCTGTAGTCGCACAGCGACTTGAAGATGTATTTGCTGG9406.480523957256118No Hit
CGTCGTATATAGTCTGTAGTCGTTGTAGTTGAATACTTGTGAGGGGTAAT440.30334367459496725No Hit
CGTCGTATATAGTCTGTAGTCGGGGGCAGGAATGAGAATACTGGAGAATT440.30334367459496725No Hit
CGTCGTATATAGTCTGTAGTCGATCAGCAAAAGCAGGTACTGATTCAAAA360.2481902792140641No Hit
CGTCGTATATAGTCTGTAGTCGATCAGCAAAAGCAGGTAGATATTGAAAG320.22061358152361252No Hit
CGTCGTATATAGTCTGTAGTCGAAACGCACGTTCTCTCTATCATCCCGTC310.21371940710099968No Hit
CGTCGTATATAGTCTGTAGTCGAGGTGACAAGACCAATCCTGTCACCTCT260.1792485349879352No Hit
CGTCGTATATAGTCTGTAGTCGATGAGCAAGGCGAATCAATAATAGTAGA260.1792485349879352No Hit
CGTCGTATATAGTCTGTAGTCGAAAGGGGAAAAAGCTAATGTGCTAATTG250.1723543605653223No Hit
CGTCGTATATAGTCTGTAGTCGAGGGCGGGAACAACTAGGTCAGAAGTTT240.1654601861427094No Hit
CGTCGTATATAGTCTGTAGTCGATCAGCAAAAGCAGGCAGATATTGAAAG230.15856601172009652No Hit
CGTCGTATATAGTCTGTAGTCGCTTCGCGGTACCTAACTGACATGACTGT220.15167183729748362No Hit
CGTCGTATATAGTCTGTAGTCGGGAGCAGGAATGAGAATACTGGAGAATT200.13788348845225784No Hit
CGTCGTATATAGTCTGTAGTCGAGGCACTTAAAATGACCATGGCCTCCGC200.13788348845225784No Hit
CGTCGTATATAGTCTGTAGTCGGGGGCGGGAACAACTAGGTCAGAAGTTT200.13788348845225784No Hit
CGTCGTATATAGTCTGTAGTCGAGTTGCTACCATGCTAACAACTCGACCG190.13098931402964495No Hit
CGTCGTATATAGTCTGTAGTCGCAAATAGGAAATATTATTTCAATATGGG180.12409513960703206No Hit
CGTCGTATATAGTCTGTAGTCGATCAGCAAAAGCAGGTAAACCATTTGAA170.11720096518441916No Hit
CGTCGTATATAGTCTGTAGTCGGGGACAGGAATGAGAATACTGGAGAATT170.11720096518441916No Hit
CGTCGTATATAGTCTGTAGTCGGGGGACTCGAACTGTGTTATCATTCCAT170.11720096518441916No Hit
CGTCGTATATAGTCTGTAGTCGATCAGCAAAAGCAGGCCAATTATATTCA160.11030679076180626No Hit
CGTCGTATATAGTCTGTAGTCGATCAGCAAAAGCAGGTCAATTATATTCA160.11030679076180626No Hit
CGTCGTATATAGTCTGTAGTCGGGGGGGGGAAGACAGGCCCCCACTGACA160.11030679076180626No Hit
CGTCGTATATAGTCTGTAGTCGCGCAGCGACTTGAAGATGTATTTGCTGG160.11030679076180626No Hit
CGTCGTATATAGTCTGTAGTCGAAACAAACAAATTTGCAGCAATATGCAC160.11030679076180626No Hit
CGTCGTATATAGTCTGTAGTCGAGAAGGGGAAAAAGCTAATGTGCTAATT160.11030679076180626No Hit
CGTCGTATATAGTCTGTAGTCGCGGTGCTCCAGTTTCGGTATTTTTTGTC160.11030679076180626No Hit
CGTCGTATATAGTCTGTAGTCGGAGGGTCACCAGCACAAAATGCTATAAG150.1034126163391934No Hit
CGTCGTATATAGTCTGTAGTCGATCAGCAAAAGCAGGCAGCAGACAAAGC150.1034126163391934No Hit
CGTCGTATATAGTCTGTAGTCGCTGCCAGAACCAATCCATTAATAAGACA150.1034126163391934No Hit
CGTCGTATATAGTCTGTAGTCGAAACAACACTGGAATATGCAACCAAAGA150.1034126163391934No Hit
CGTCGTATATAGTCTGTAGTCGAGGACCCAAATGCACTTTTAAAGCACAG150.1034126163391934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA13850.094.6369861
ATAGTCT14200.093.9930659
TATAGTC14150.093.989528
CGTATAT14000.093.978734
TCGTATA14000.093.978733
GTCGTAT13900.093.971412
ATATAGT14150.093.653847
GTATATA14050.093.644295
TATATAG14000.093.639466
AGTCGGC650.047.4982818-19
CGGAGGC205.3021905E-447.49827620-21
GTCGCGG501.0913936E-1147.49827618-19
GTCGCGA205.3021905E-447.49827618-19
CGGGGGA357.570634E-847.49827620-21
GTCGTGA301.4425568E-647.49827618-19
GTCGCAA452.1100277E-1047.49827618-19
TCGAAGG403.985406E-947.49827620-21
TCGAAGA205.3021905E-447.49827620-21
TCGCGGG403.985406E-947.49827620-21
GTCGATG252.7594699E-547.49827618-19
Produced by FastQC (version 0.11.5)