FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC117CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC117CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23750
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTATCACTACTAGTACTCTCAAACCCCTTTCTAAGTCTGTTACTTTTG1850.7789473684210526No Hit
CGCTATCACTACTAGTACTCTCGGGGGACTCGAACTGTGTTATCATTCCA1520.64No Hit
CGCTATCACTACTAGTACTCTCAACCCAGAAGCAAGGTCTTATACAGTCC1310.5515789473684211No Hit
CGCTATCACTACTAGTACTCTCATGCCCAAGCAAAAAGTGGCTGGCCCTC1160.488421052631579No Hit
CGCTATCACTACTAGTACTCTCAATCCAAACTGGAAGTCAAAACAACACT800.3368421052631579No Hit
CGCTATCACTACTAGTACTCTCCGGCAACAACATTAGTGTTGTTAATCAT730.30736842105263157No Hit
CGCTATCACTACTAGTACTCTCAAGCCCCTTTCTAAGTCTGTTACTTTTG650.27368421052631575No Hit
CGCTATCACTACTAGTACTCTCGGGGGGCAAAGACATAATGGATTCCCAC650.27368421052631575No Hit
CGCTATCACTACTAGTACTCTCAGCCCAGAAGCAAGGTCTTATACAGTCC630.2652631578947368No Hit
CGCTATCACTACTAGTACTCTCCGGCAACAACATTAGTGTTGTTAATATT560.23578947368421052No Hit
CGCTATCACTACTAGTACTCTCAATCCCAACTGGAAGTCAAAACAACACT520.21894736842105264No Hit
CGCTATCACTACTAGTACTCTCCGGCAACAACACTAATGTTGTTGCTGGA490.20631578947368423No Hit
CGCTATCACTACTAGTACTCTCACAAGCTACAAACTGAGAGATAATATTT490.20631578947368423No Hit
CGCTATCACTACTAGTACTCTCAATCGAAACTGGAAGTCAAAACAACACT490.20631578947368423No Hit
CGCTATCACTACTAGTACTCTCAGAAGCAAGGTCTTATACAGTCCAATCC480.20210526315789473No Hit
CGCTATCACTACTAGTACTCTCCTGCAACAACACTAATGTTGTTGCTGGA480.20210526315789473No Hit
CGCTATCACTACTAGTACTCTCAAGCCAAACTGGAAGTCAAAACAACACT470.1978947368421053No Hit
CGCTATCACTACTAGTACTCTCGATATTCTTCCCTCATAGACTCTGGTAC460.1936842105263158No Hit
CGCTATCACTACTAGTACTCTCAGACGGCCAAATTCCTCATTTTTGCCGT460.1936842105263158No Hit
CGCTATCACTACTAGTACTCTCAGACCCCTTTCTAAGTCTGTTACTTTTG370.15578947368421053No Hit
CGCTATCACTACTAGTACTCTCCAGCAACAACATTAGTGTTGTTAATATT350.1473684210526316No Hit
CGCTATCACTACTAGTACTCTCAACCGAGAAGCAAGGTCTTATACAGTCC320.13473684210526315No Hit
CGCTATCACTACTAGTACTCTCGGGGGGGCAAAGACATAATGGATTCCCA290.12210526315789474No Hit
CGCTATCACTACTAGTACTCTCCCGCAACAACACTAATGTTGTTGCTGGA290.12210526315789474No Hit
CGCTATCACTACTAGTACTCTCAAGCCAGAAGCAAGGTCTTATACAGTCC280.11789473684210526No Hit
CGCTATCACTACTAGTACTCTCCATCAACAGTCACTGGATTGCAGCTGCC270.11368421052631579No Hit
CGCTATCACTACTAGTACTCTCAGTCCAAACTGGAAGTCAAAACAACACT270.11368421052631579No Hit
CGCTATCACTACTAGTACTCTCAGCACCGTCTGGCCAAGACCAGTTTGCA260.10947368421052632No Hit
CGCTATCACTACTAGTACTCTCAACCCCGAAGCAAGGTCTTATACAGTCC250.10526315789473684No Hit
CGCTATCACTACTAGTACTCTCAGGGGGCAAAGACATAATGGATTCCCAC250.10526315789473684No Hit
CGCTATCACTACTAGTACTCTCAGGCTCTACTATTATTGATTCGCCTTGC250.10526315789473684No Hit
CGCTATCACTACTAGTACTCTCCCAAGCAAAAAGTGGCTGGCCCTCTTTG250.10526315789473684No Hit
CGCTATCACTACTAGTACTCTCGGGGGGCGAAGACATAATGGATTCCCAC240.10105263157894737No Hit
CGCTATCACTACTAGTACTCTCAGGCCCCCTCAAAGCCGAGATCGCACAG240.10105263157894737No Hit
CGCTATCACTACTAGTACTCTCAGAACCCAAATCAAAAGATAATAACCAT240.10105263157894737No Hit
CGCTATCACTACTAGTACTCTCAAAGACTCCCCGCCCCTGGAAGGACACT240.10105263157894737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA254.481899E-795.08
CTCCTAG156.537717E-495.09
ACTCCTA156.537717E-495.08
TATCAAT254.481899E-795.04
ATACTAG301.1805241E-895.09
CTACTCG156.537717E-495.09
CCCTACT254.481899E-795.07
ACTACTC156.537717E-495.08
CGCTGTC254.481899E-795.01
CTATCAA254.481899E-795.03
CTACTAG22450.094.7884149
CACTACT22350.094.7874767
ACTACTA22100.094.7850658
TCACTAC22600.094.1592946
CGCTATC23400.093.782051
GCTATCA22950.093.758172
CTATCAC22650.093.741723
TATCACT22800.093.333334
ATCACTA22800.093.1255
GTCACTA301.3264271E-679.166675
Produced by FastQC (version 0.11.5)