FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC106CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC106CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32469
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTATACGTCACGTGCTGTACCCCGACCAATCAGTTATTGCTACAACA1500.46197911854384177No Hit
CGACTATACGTCACGTGCTGTAGGAGGGGCAAGAGTTCTGCTGAGGACAC1280.3942221811574117No Hit
CGACTATACGTCACGTGCTGTACCCAGACCAATCAGTTATTGCTACAACA1030.3172256614001047No Hit
CGACTATACGTCACGTGCTGTACCCTGACCAATCAGTTATTGCTACAACA940.2895069142874742No Hit
CGACTATACGTCACGTGCTGTACGCGTGATCAGTAGAAACAAGGTCGTTT600.18479164741753673No Hit
CGACTATACGTCACGTGCTGTAACGAAAACGTTCTAATCTCTTGTTCCAC500.15399303951461393No Hit
CGACTATACGTCACGTGCTGTAGAGGGCAGCTGCAATCCAGTGACTGTTG470.1447534571437371No Hit
CGACTATACGTCACGTGCTGTAGGAAGGGGAAAAAGCTAATGTGCTAATT470.1447534571437371No Hit
CGACTATACGTCACGTGCTGTAGAAAGGGGAAAAAGCTAATGTGCTAATT420.12935415319227572No Hit
CGACTATACGTCACGTGCTGTACCCCCATTCGTTTCTGATAGGCCTGTAA410.12627429240198343No Hit
CGACTATACGTCACGTGCTGTAAGGGGAAAAAGCTAATGTGCTAATTGGG410.12627429240198343No Hit
CGACTATACGTCACGTGCTGTAAAGGGGAAAAAGCTAATGTGCTAATTGG400.12319443161169115No Hit
CGACTATACGTCACGTGCTGTAGATGGAGCAAACGGAGTAAAAGGGTTTT400.12319443161169115No Hit
CGACTATACGTCACGTGCTGTAAAAGGGGAAAAAGCTAATGTGCTAATTG390.12011457082139888No Hit
CGACTATACGTCACGTGCTGTAGGAGGGCCAAGAGTTCTGCTGAGGACAC380.11703471003110659No Hit
CGACTATACGTCACGTGCTGTAATCCCCTTTTCTGTCTTGATAAGTCTGG380.11703471003110659No Hit
CGACTATACGTCACGTGCTGTACTGACAGCAATCTACTCCCCTGGAATTC370.11395484924081431No Hit
CGACTATACGTCACGTGCTGTAGGGGGGCAAACGGAGTAAAAGGGTTTTC350.10779512766022976No Hit
CGACTATACGTCACGTGCTGTAGGGGGGGCAACGAGATCCATTCTGTGGT340.10471526686993747No Hit
CGACTATACGTCACGTGCTGTAGGGGGAAAAAGCTAATGTGCTAATTGGG340.10471526686993747No Hit
CGACTATACGTCACGTGCTGTACCAACACTACATCCCCTTGCCCAATTAG330.1016354060796452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAT650.095.1450651
CGATTAT850.095.1450651
CGGCTAT650.095.1450651
ACTATCC201.7184406E-594.998463
ATAGGTC254.5200068E-794.998466
CTATCCG201.7184406E-594.998464
CGGCACG254.5200068E-794.998469
ACTCTAC254.5200068E-794.998463
GCTATAC650.094.998463
TACGGCA254.5200068E-794.998467
AATATAC201.7184406E-594.998463
ATCCGTC201.7184406E-594.998466
TTATACG800.094.998464
ACGGCAC254.5200068E-794.998468
CTACGTC254.5200068E-794.998466
CACTATA353.1650416E-1094.998462
ATATACG201.7184406E-594.998464
TCCGTCA201.7184406E-594.998467
ACGTCAT353.1650416E-1094.998468
ACGTCAA254.5200068E-794.998468
Produced by FastQC (version 0.11.5)