FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC099CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC099CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112217
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGTCTACTACTCTACTGTTCCTCTCGATATTCTTCCCTCATAGACT27732.4711050910289885No Hit
CGTACGTCTACTACTCTACTGTATGAGCTACTTGGGAAGAATTTTTCAAA14281.2725344644750796No Hit
CGTACGTCTACTACTCTACTGTAGGCCACATGGCCATAATCAAGAAATAC10400.9267758004580412No Hit
CGTACGTCTACTACTCTACTGTCTTCCCGATGTGTATTTCTTGATTATGG5460.48655729524047164No Hit
CGTACGTCTACTACTCTACTGTAGACCACATGGCCATAATCAAGAAATAC5190.4624967696516571No Hit
CGTACGTCTACTACTCTACTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC4850.432198330021298No Hit
CGTACGTCTACTACTCTACTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG4270.38051275653421496No Hit
CGTACGTCTACTACTCTACTGTCTTCCTGATGTGTATTTCTTGATTATGG4260.37962162595685145No Hit
CGTACGTCTACTACTCTACTGTAGCCCACATGGCCATAATCAAGAAATAC2750.24506090877496278No Hit
CGTACGTCTACTACTCTACTGTCGGCAACCTGTGAACAGATTGCCGACTC2350.20941568568042276No Hit
CGTACGTCTACTACTCTACTGTAGCCACATGGCCATAATCAAGAAATACA2270.20228664106151473No Hit
CGTACGTCTACTACTCTACTGTAATGCTTGCATAAATGTTATTTGCTCAA2220.19783098817469724No Hit
CGTACGTCTACTACTCTACTGTGAGGGCGAAACGTACGTTCTCTCTATCA1900.1693148096990652No Hit
CGTACGTCTACTACTCTACTGTCACCCCTGACTAAGGGCATTTTGGACAA1750.15594785103861267No Hit
CGTACGTCTACTACTCTACTGTAGCCCACATTGCCATCATCCATTTCATC1740.1550567204612492No Hit
CGTACGTCTACTACTCTACTGTGGGGGGCTATATGAAGCAATTGAGGAGT1740.1550567204612492No Hit
CGTACGTCTACTACTCTACTGTAACCACATGGCCATAATCAAGAAATACA1740.1550567204612492No Hit
CGTACGTCTACTACTCTACTGTCAGAAACGAATGGGGGTGCAGATGCAAC1650.14703654526497767No Hit
CGTACGTCTACTACTCTACTGTGGGGCCGCAAGTATAATTGGGATTGTGC1620.14436315353288717No Hit
CGTACGTCTACTACTCTACTGTAATTGGGTATTTCATTGCCATCATCCAT1560.13901637006870615No Hit
CGTACGTCTACTACTCTACTGTATGAACGGGCCATCGGTCATTATGGTGA1490.13277845602716168No Hit
CGTACGTCTACTACTCTACTGTCCTCGCTCACTGGGCACGGTGAGCGTGA1420.12654054198561715No Hit
CGTACGTCTACTACTCTACTGTCGGCCCCCTCAAAGCCGAGATCGCACAG1320.11762923621198214No Hit
CGTACGTCTACTACTCTACTGTCGTCCCGTCAGGCCCCCTCAAAGCCGAG1290.11495584447989164No Hit
CGTACGTCTACTACTCTACTGTCGACCACATGGCCATAATCAAGAAATAC1290.11495584447989164No Hit
CGTACGTCTACTACTCTACTGTGATGGGAATGATTGGGATATTACCTGAT1240.11050019159307414No Hit
CGTACGTCTACTACTCTACTGTGAAAGGGGAAAAAGCTAATGTGCTAATT1240.11050019159307414No Hit
CGTACGTCTACTACTCTACTGTCTCCTCTGACTAAGGGCATTTTGGACAA1240.11050019159307414No Hit
CGTACGTCTACTACTCTACTGTCAGGAACGAATGGGGGTGCAGATGCAAC1200.10693566928362012No Hit
CGTACGTCTACTACTCTACTGTAAAAGGGGAAGGTTACTAAATCAATAGA1170.10426227755152963No Hit
CGTACGTCTACTACTCTACTGTTTCGGTATTTTTTGTCCATCTTCCTCTT1160.10337114697416612No Hit
CGTACGTCTACTACTCTACTGTAAACCACATGGCCATAATCAAGAAATAC1150.10248001639680263No Hit
CGTACGTCTACTACTCTACTGTGGGGCGGGAACAACTAGGTCAGAAGTTT1150.10248001639680263No Hit
CGTACGTCTACTACTCTACTGTACGCGTGATCAGCAAAAGCAGGTCAATT1140.10158888581943912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGT600.095.083851
ATACTAC201.7370294E-594.9991158
TCTCCTA450.094.9991157
TACTACC201.7370294E-594.9991159
TAATACT254.593603E-794.999119
CTACTAA254.593603E-794.999118
GCACGTC254.593603E-794.999112
CGTCTAG254.593603E-794.999115
CGTCTAA254.593603E-794.999115
TTACTAC156.6024833E-494.999118
ACGGCTA500.094.999114
GCCTACT700.094.999116
CGGCTAC500.094.999115
TACTAGT254.593603E-794.999119
TACTACA254.593603E-794.999119
GTAAGTC301.2218152E-894.999112
ACGACTA254.593603E-794.999114
CTAATAC254.593603E-794.999118
TGCGTCT301.2218152E-894.999113
ACGTCTA107450.094.114994
Produced by FastQC (version 0.11.5)