FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC082CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC082CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78365
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGATCGTCTCATACGTAGCCCCCCAAGATCAAAGGTCCCAGGTTCCA4810.613794423530913No Hit
CGTAGATCGTCTCATACGTAGCAGGCACTGATTCAAAATGGAAGATTTTG4760.6074140241179098No Hit
CGTAGATCGTCTCATACGTAGCCCCCGAAGATCAAAGGTCCCAGGTTCCA4000.5104319530402603No Hit
CGTAGATCGTCTCATACGTAGCCCACCAAGATCAAAGGTCCCAGGTTCCA3050.3892043641931985No Hit
CGTAGATCGTCTCATACGTAGCCCCACAAGATCAAAGGTCCCAGGTTCCA2600.3317807694761692No Hit
CGTAGATCGTCTCATACGTAGCCGCCCAAGATCAAAGGTCCCAGGTTCCA2600.3317807694761692No Hit
CGTAGATCGTCTCATACGTAGCCCCCAAGATCAAAGGTCCCAGGTTCCAG2570.32795252982836726No Hit
CGTAGATCGTCTCATACGTAGCCCCCTAAGATCAAAGGTCCCAGGTTCCA2440.31136349135455876No Hit
CGTAGATCGTCTCATACGTAGCCACCCAAGATCAAAGGTCCCAGGTTCCA1990.25393989663752947No Hit
CGTAGATCGTCTCATACGTAGCGAAGGGGGAAAAAGCTAATGTGCTAATT1890.241179097811523No Hit
CGTAGATCGTCTCATACGTAGCAGGACACTGTTGGCTAAGTCAGTATTCA1890.241179097811523No Hit
CGTAGATCGTCTCATACGTAGCCCTCCAAGATCAAAGGTCCCAGGTTCCA1880.23990301792892235No Hit
CGTAGATCGTCTCATACGTAGCCTCCCAAGATCAAAGGTCCCAGGTTCCA1840.23479869839851975No Hit
CGTAGATCGTCTCATACGTAGCACCCCAAGATCAAAGGTCCCAGGTTCCA1800.2296943788681171No Hit
CGTAGATCGTCTCATACGTAGCGGAAGGGGAAAAAGCTAATGTGCTAATT1610.2054488610987048No Hit
CGTAGATCGTCTCATACGTAGCAGGCCAATTATATTCAATATGGAAAGAA1580.20162062145090281No Hit
CGTAGATCGTCTCATACGTAGCAGGCCCTCTTTTTAAACCGTGTTTAAAG1560.1990684616857015No Hit
CGTAGATCGTCTCATACGTAGCGGGAGGGGAAAAAGCTAATGTGCTAATT1540.19651630192050024No Hit
CGTAGATCGTCTCATACGTAGCAGAAAAAGCAATGAAAGAGTATGGAGAG1530.19524022203789956No Hit
CGTAGATCGTCTCATACGTAGCGAGGGGGGAAAAAGCTAATGTGCTAATT1470.18758374274229567No Hit
CGTAGATCGTCTCATACGTAGCCCGCCAAGATCAAAGGTCCCAGGTTCCA1390.17737510368149048No Hit
CGTAGATCGTCTCATACGTAGCCTAAAATGAGGAATCCCCTTAGAACCGC1380.1760990237988898No Hit
CGTAGATCGTCTCATACGTAGCGGAGGGGGAAAAAGCTAATGTGCTAATT1370.17482294391628916No Hit
CGTAGATCGTCTCATACGTAGCGAAAGGGGAAAAAGCTAATGTGCTAATT1370.17482294391628916No Hit
CGTAGATCGTCTCATACGTAGCAGCAAAAGCAGGTACTGATTCAAAATGG1370.17482294391628916No Hit
CGTAGATCGTCTCATACGTAGCAAAAGCAGGTACTGATTCAAAATGGAAG1350.17227078415108785No Hit
CGTAGATCGTCTCATACGTAGCGAGAGGGGAAAAAGCTAATGTGCTAATT1290.16461430485548395No Hit
CGTAGATCGTCTCATACGTAGCAAAGGGGAAAAAGCTAATGTGCTAATTG1060.13526446755566898No Hit
CGTAGATCGTCTCATACGTAGCAAAAGGGGAAAAAGCTAATGTGCTAATT1060.13526446755566898No Hit
CGTAGATCGTCTCATACGTAGCAGGTACTGATTCAAAATGGAAGATTTTG1020.1301601480252664No Hit
CGTAGATCGTCTCATACGTAGCCCCCCCAGATCAAAGGTCCCAGGTTCCA1000.12760798826006509No Hit
CGTAGATCGTCTCATACGTAGCGCAAGGGGAAAAAGCTAATGTGCTAATT970.12377974861226312No Hit
CGTAGATCGTCTCATACGTAGCGGAGGGGAAAAAGCTAATGTGCTAATTG950.12122758884706183No Hit
CGTAGATCGTCTCATACGTAGCGGGGGGGGAAAAAGCTAATGTGCTAATT930.11867542908186052No Hit
CGTAGATCGTCTCATACGTAGCAAAGGGGGAAAAGCTAATGTGCTAATTG900.11484718943405856No Hit
CGTAGATCGTCTCATACGTAGCAGAAGGGGAAAAAGCTAATGTGCTAATT880.11229502966885728No Hit
CGTAGATCGTCTCATACGTAGCAGGCGCTGATTCAAAATGGAAGATTTTG870.11101894978625662No Hit
CGTAGATCGTCTCATACGTAGCGAAGGGGAAAAAGCTAATGTGCTAATTG870.11101894978625662No Hit
CGTAGATCGTCTCATACGTAGCCAAAGGGGAAAAAGCTAATGTGCTAATT870.11101894978625662No Hit
CGTAGATCGTCTCATACGTAGCGAGGGGGAAAAAGCTAATGTGCTAATTG860.10974286990365596No Hit
CGTAGATCGTCTCATACGTAGCAGGCAAACCATTTGAATGGATGTCAATC840.10719071013845466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGAT1150.095.180311
CGTATAT500.095.1803051
CGTACAT156.545167E-495.1803051
AAATCGT500.094.9980854
GGTCGTC800.094.9980855
GATCGAC301.2170858E-894.9980855
TAGACCG254.5808702E-794.9980853
TATATCG500.094.9980853
CATCTCA254.5808702E-794.9980858
CGTCTCT156.59526E-494.9980858
CGTCTCG550.094.9980858
CGTCTAA353.255991E-1094.9980858
TACATCG156.59526E-494.9980853
ATATCGT500.094.9980854
GTCACAT156.59526E-494.9980859
AGACCGT254.5808702E-794.9980854
TCGTCTG409.094947E-1294.9980857
TCGTCTA353.255991E-1094.9980857
GAACGTC600.094.9980855
GTATATC500.094.9980852
Produced by FastQC (version 0.11.5)