FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC070CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC070CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGAGCTACGTCGTGCACGTAGGGGGGAGAAGCTTTCAGTCTCGTACCA60.303951367781155No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGCAAGAAGGGGATTGAACTCAG50.25329280648429586No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGAAATGAAGCAGGGGGCTGCGC40.2026342451874367No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGCAAGATTATTAGCGGTTTTGC40.2026342451874367No Hit
CGTGAGCTACGTCGTGCACGTAAACCGCTAATAATCTTGCCGCCAACTAC40.2026342451874367No Hit
CGTGAGCTACGTCGTGCACGTACAAGTGGCACACACTAGACCAAAAGCAG40.2026342451874367No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGCCAAGGAGGTGTCACTAAGCTA40.2026342451874367No Hit
CGTGAGCTACGTCGTGCACGTAGGAGGGGCAAAACCGCTAATAATCTTGC30.1519756838905775No Hit
CGTGAGCTACGTCGTGCACGTACAGACCCAGACTTAGGAGTGTCCTGATA30.1519756838905775No Hit
CGTGAGCTACGTCGTGCACGTAAGGGCGGCAAGATTATTAGCGGTTTTGC30.1519756838905775No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGCTGCGCGCGGCGTAGAAAACTGC30.1519756838905775No Hit
CGTGAGCTACGTCGTGCACGTAGGCAAGAGAAGAACCCCGCACTCAGAAT30.1519756838905775No Hit
CGTGAGCTACGTCGTGCACGTAGGGGAGGCAAGAGAAGAACCCCGCACTC30.1519756838905775No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGCTAACCCTAATGGCAAGGACTA30.1519756838905775No Hit
CGTGAGCTACGTCGTGCACGTAGAAAAGCGAAAGCATAGAAAGTGAGTGT30.1519756838905775No Hit
CGTGAGCTACGTCGTGCACGTAAAGGAGAGAAAGCTAATGTACTAATTGA20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAATGACCCCGCCACCCAAGATAAAGCGTT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGAGGAGCAATTGAATTAGGCGGTACAA20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGAAGAAGGGGATTGAACTCAGCAA20.10131712259371835No Hit
TGTGAGCTACGTCGTGCACGTAGAAAAGGGAAGGCTCAGGTGTGTGCAGA20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGCGGAGAAAACCGCTAATAATCTTGCC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACGCAAAATCAAATAATAGTTGTAAAGTG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGAAGGTGGCACTGTTGGCTTCCCCCTTT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAAACCCCATCTCTACTAAAAATACAAAA20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGCAAGGGGAAATGAAGCAGGGGGCTGCG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAAAGAGAGAACACTGCTACTGATCCTAT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGGAAGCTTCGTGCTGGAGAAAA20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAGGGGGGCAAGTGTGAGTGAATGTGTGT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTATTGGGAAGAGTTGTCGTTGCTTCATGAC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTATGGCCATAATCAAAAAGTACACATCAGG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGACAGCAAAAGCAGGTTTCCTTGTGT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACAGGAGGCAAGAGAAGAACCCCGCACTC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAACAAGGGAAGAAGAAAAGCGAAAGCAT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACAACGGGGAATTCTTGAGGATGAACAGA20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAACGGGGGGAATCAATAATTGTAGAATCT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTATTTGGGCATTAATTTCTTTACTTGTTGT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAATTTGCAGGCCTACCAGAAGCGAATGGG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGAAGGGAAGAGAAGAACCCCGCACTC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACAGAAAGGAATGAACAAGGACAAACCCT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACCAAAAGCAAATAATAGTTGTAAAGTGC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGGCGAGAAGGGGATTGAACTCAGCAA20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAGGAAGGGAAGAGAAGAACCCCGCACTC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGAAAGCGAAAGCATAGAAAGTGAGTGT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGCAGGCGGAACGATGAAACCTTCTAATT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAGCAGGGGAATTGGGAGAGCCTAAGTTG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGAGGCGGCAAGATTATTAGCGGTTTTGC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGACCGGAACAACATGGATAGAGCAGT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAAAGGTGGCACTGTTGGCTTCCCCCTTT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGAGGCCAAGTGACTTTTTATGACTTG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGCCAGGGGAAATGAAGCAGGGGGCTGCG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGCCAAATATATTCAATATGGAGAGAAT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAGGGGGAGAAGCTTTCAGTCTCGTACCA20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACACAAAATCAAATAATAGTTGTAAAGTG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAACACTCATATTCACACACACCCATTCAC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAAAGGGAGAAGACACAAATTACTAAAAT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGGAGAGAATAAAAGAACTGAGAGATC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTATTGGTGATATAACCATAGTCCTTGCCAT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGGGGGCAAAACCGCTAATAATCTTGC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGAAGGCAAGAGAAGAACCCCGCACTC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGGGGCGGCAAGATTATTAGCGGTTTTGC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAGAAGAAAAGCGAAAGCATAGAAAGTGAG20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACAAGAAGGAATTTGATGTACCAATGGCT20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTAAGGAAGGCAAGAGAAGAACCCCGCACTC20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACAGGGGAGAAGCTTTCAGTCTCGTACCA20.10131712259371835No Hit
CGTGAGCTACGTCGTGCACGTACCAACACTACGTCCCCTTGCCCAATTAG20.10131712259371835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGCT1700.095.000012
GGTGAGC155.663022E-495.01
ACGTCGT1900.095.09
AGCTACG1900.095.05
GAGCTAC1850.095.04
CTACGTC1900.095.07
TGAGCTA1800.095.03
TACGTCG1950.094.999998
CGTGAGC1500.094.999991
GCTACGT1950.092.5640956
CACGTAG850.047.50000416-17
AGGGGGG150.00957628147.522-23
GTCGTGC1900.047.510-11
CACGTAA451.2732926E-1047.516-17
GGCAAGA150.00957628147.528-29
ACGTAGG650.047.518-19
ACGTAAA150.00957628147.518-19
CGTAGGC150.00957628147.518-19
TCGTGCA1900.047.512-13
GCACGTA1750.047.516-17
Produced by FastQC (version 0.11.5)