FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC060CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC060CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGAGCGAGACAGTCGTGTCGAAAAAGGCAAGAGAATTGCCTCTTACCA11.9607843137254901No Hit
CACGAGCGAGACAGTCGTGTCGAACAGGCCAAATATATTCAATATGGAGA11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGAAAAGGAAAGAAAGCTAATGTGCTAAT11.9607843137254901No Hit
CTCGAGCGAGACAGTCGTGTCGGAAAACAGAAAGCCGATACAGAAAAACA11.9607843137254901No Hit
CGCTAGCGAGACAGTCGTGTCGATTGAATGCTGCCCGGGGGATTAGGGAC11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGTAGGCCCGCAAATTTTCAAGAAGGTCAT11.9607843137254901No Hit
CGCGAGCTAGACAGTCGTGTCGCTAACAATTCGACACTAATTGATGGCCA11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGAAAACAGGATTGCTCAGTTCCTTGTTCA11.9607843137254901No Hit
CGTGAGCGAGACAGTCGTGTCGGCGAAAGCAAGTGACAAAAACATAATGG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGAAAGGATGCAGGTGCATTGACTGAAGA11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGCAGCCCCCAAAATCTGTATTCAACAGTC11.9607843137254901No Hit
TGCGAGCGAGTCAGTCGTGTCGGTAGGCCTGCAAATTTTCAAGAAGGTCA11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGAAAACAATTCGACACTAATTGATGGCCA11.9607843137254901No Hit
CGTGAGCGAGACAGTCGTGTCGGACAAGAGAATAATGAAGACAAGAGATA11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGAAAAGAGGAACCCAGCGAGACACTTAGA11.9607843137254901No Hit
CGAGAGCGAGACAGTCGTGTCGAAGAAACAAGGTCGTTTTTAAACAATTC11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGAAAAGCAGGTTAAGCAAGGTTTTAAGGT11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGAGGACAGGACTGCTCAAATGAGTATAGG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGCAGGAACAAGGAATATTGAAGCTCTGA11.9607843137254901No Hit
CGGGAGCGAGACAGTCGTGTCGCCAAGAGGAAAGCTTTCCTCTTGGAATC11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGCCGGCCCAAATTGAAAGCGACAGAGAAT11.9607843137254901No Hit
TGCGAGCGAGACAGTCGTGTCGCAGGGGGGAAGCTTTCAGTCTCGTACCA11.9607843137254901No Hit
CGTGAGCGAGACAGTCGTGTCGGACGGGCCAACATGGTGAAACCCCATCT11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGGGGGCCCGAAAACAGAAGGGACCCCAA11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGGCGCAGACCAGCACTCGAGATCAACTCAATA11.9607843137254901No Hit
CGAGAGCGAGACAGTCGTGTCGGCGGAGCAGATAGGGAGGTCAGAAGAGA11.9607843137254901No Hit
TGCGAGCGAGACAGTCGTGTCGAACACAACAATACTCAGAAAAGGGAAAG11.9607843137254901No Hit
CGCGAGCGCGACAGTCGTGTCGGAGGGGGCAAGAGAAGAACCCCGCACTC11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGCGGCAGGAAGGCAAGAGAAGAACCCCG11.9607843137254901No Hit
CGTGAGCGAGACAGTCGTGTCGATGTCGAAACAGCCACTCTTGTTGGGAA11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGCCGGAAGGAAGAAGGCGAGCATTGTCAGTGT11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGCACACAGGAAACCTCCTTGCCCAGTAAA11.9607843137254901No Hit
CGAGAGCGAGACAGTCGTGTCGGAACAGGCAATGAAAGAATATGGGGAAG11.9607843137254901No Hit
CGTGAGCGAGACAGTCGTGTCGCCAACAGTCTATAAGCATTCCCTTTTCT11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGGAAAACAAAAGCAACAAAAATGAAGGG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGTAAGTATGCTAGAGTCCCGTTTTCGTT11.9607843137254901No Hit
CGCGAGCGAGACAGGCGTGTAGAGGCCGAGAGGGAAAAGAAAAGAGATGG11.9607843137254901No Hit
CGGGAGCGAGACAGTCGTGTCGGTAACCCCCTGTCCAGGAGAAACAACGG11.9607843137254901No Hit
CGGGAGCGAGACAGTCGTGTCGAGGGGGCCAAGGAGGTGTCACTAAGCTA11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGCAAAAGCAGGCTAGGGTGAAAAATGAA11.9607843137254901No Hit
CGCGAGCGAGACAGTTGTGTCGGAGGGGGGAGAAAAATTGGACAAACAGC11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGCAGGCCAAATATATTCAATATGGAGAG11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGTTCTGATAGAACTGCAACAATGATGAGT11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGGAGGGGGGGGAAACTGGATAACGGGGGCGCT11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGAAAGGGGGAACATTCATAATGTTTTCTT11.9607843137254901No Hit
AGCGAGCGAGACAGTCGTGTCGTCCAGGCCAAGATTCGGTCTCTTCCTTC11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGGGCAGGGGAGAACCCCGCCCTCAGAAT11.9607843137254901No Hit
AGCGAGCGAGACAGTCGTGTCGAAAAGAGCAAGACTTGGAAATCAGGAGG11.9607843137254901No Hit
CACGAGCGAGACAGTCGTGTCGGGGGAGGCAAGAGAAGCACCCCGCACCC11.9607843137254901No Hit
CGCGAGCGAGACAGTCGTGTCGGGAAAGAGCGCGAGCGAGACAGTCGTGT11.9607843137254901No Hit
CGTGAGCGAGACAGTCGTGTCGGGGGACCGAATGGCCATAAACAAAAAGG11.9607843137254901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAG50.094.999994
CGAGCGA50.094.999993
CGAGACA50.094.999997
AGCGAGA50.094.999995
AGACAGT50.094.999999
GCGAGCG50.094.999992
GCGAGAC50.094.999996
GAGACAG50.094.999998
CGCGAGC50.094.999991
Produced by FastQC (version 0.11.5)