FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC050CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC050CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20685
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGAGTACACACTACGTAGCAAGGAGCTTGCTTCTGTAGTGCTGTGTTG620.29973410684070584No Hit
CGAGAGTACACACTACGTAGCAAGGCGATCTTCCAGGAGCTAATGGTAGT410.1982112642011119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGGT407.2759576E-1295.228161
CGAGCGT156.463321E-495.228161
CGTGAGT254.3972432E-795.228161
GGGAGTA254.4615808E-794.997592
AGTACGC254.4615808E-794.997595
TACGCAC254.4615808E-794.997597
GAGTACG254.4615808E-794.997594
TGAGTAC254.4615808E-794.997593
GGAGTAC254.4615808E-794.997593
GTACGCA254.4615808E-794.997596
GAGCGTA156.5261335E-494.997582
TACATAC301.1728844E-894.997587
GAGTACC156.5261335E-494.997584
CGTACAC156.5261335E-494.997585
TAGAGTA156.5261335E-494.997582
GCGTACA156.5261335E-494.997584
AGCGTAC156.5261335E-494.997583
CATACTA301.1728844E-894.997589
AACACTA301.1728844E-894.997589
GTAGACA201.7036271E-594.9975746
Produced by FastQC (version 0.11.5)