FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC047CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC047CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1639
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTCATAGTGTACGTCGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTT80.4881025015253203No Hit
CGCTCATAGTGTACGTCGACTCAGGCGCTCCTTCCGTAGAAGGCCCTCTT50.3050640634533252No Hit
CGCTCATAGTGTACGTCGACTCTGGTTGTTAGTCCATTGTAATACTTTGC40.24405125076266015No Hit
CGCTCATAGTGTACGTCGACTCAGGCCCTCCTTCCGTAGAAGGCCCTCTT40.24405125076266015No Hit
CGCTCATAGTGTACGTCGACTCCACCCCAGATGTGCCTTCATCTGGATCT30.18303843807199513No Hit
CGCTCATAGTGTACGTCGACTCATTCCCATTTAGGGCATTTTGGATAAAG30.18303843807199513No Hit
CGCTCATAGTGTACGTCGACTCCAGAGCAGAAATGAAAAGTGGCGAGAGC30.18303843807199513No Hit
CGCTCATAGTGTACGTCGACTCAAGGGACAAAGCTGTTTGAGAGAGGAAA30.18303843807199513No Hit
CGCTCATAGTGTACGTCGACTCGAGGCCAAGGGTGTTGCCTCTTCCTTTT30.18303843807199513No Hit
CGCTCATAGTGTACGTCGACTCAGGCCAAACCTAACAAGGCTAGTCTTAC30.18303843807199513No Hit
CGCTCATAGTGTACGTCGACTCCCAGAAGCAAATGGGAGTGCAGATGCAG20.12202562538133008No Hit
AGCTCATAGTGTACGTCGACTCAGGAACTCCTTCCGTAGAAGGCCCTCTT20.12202562538133008No Hit
CGCTAATAGTGTACGTCGACTCGTCATTGCAGCAAATATCATTGGGATCT20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCAGAAGCGAATGGGAGTGCAGATGCAGC20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCATCACATATTTACAGAAGAAATAACAG20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCCCCCAAAGCCAAAATAACCAGGAAAAA20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCAACGCCATGTCAAGCTTTCAGGTAGAC20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCAACGGGGAATTCTTGAGGATGAACAGA20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCGGACAGGGAAGATGGAGTTGGTGACTCA20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCAACCGATTAGAGACCTTGATACTACTAA20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCAGAAGGGAATGGGAGTGCAGATGCAGC20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCAACCAAGCAGAAGGAAGATAATATAAA20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCAGAAGCGAATGGGAGTGCAGATGCAGCG20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCACAACCCCAACCAAGCAGAAGGAAGATA20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCCGAAGCGAATGGGAGTGCAGATGCAGC20.12202562538133008No Hit
AGCTCATAGTGTACGTCGACTCGCGAAGGCAATGAAAGAATATGGGAAGA20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCCGGGACAAAGCTGTTTGAGAGAGGAAA20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCTGACCACTCATTTATTCCTACGATATC20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCAGGGGATACAAAATGGATACACAGATAA20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCGTGCCTTTGAGAAGGTTATGAGCAATGA20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCAGCCTTGTTAATCACTAGAGATGCAATA20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCCGCTATATCCTGACCACTCATTTATTC20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCAGTAGAAACAAGGTAGTTTTTTACTCTA20.12202562538133008No Hit
CGCTCATAGTGTACGTCGACTCCAACCCCAACCAAGCAGAAGGAAGATAA20.12202562538133008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTA1450.095.07
GTGTACG1350.095.09
CGCTCAT1250.095.01
ATAGTGT1450.095.06
AGTGTAC1350.095.08
CATAGTG1500.095.05
CTCATAG1450.095.03
GCTCATA1400.095.02
TCATAGT1550.095.04
TCGACTC1450.047.516-17
ACGTCGA1450.047.512-13
TCAGGAA150.00941632647.520-21
GTCGACT1450.047.514-15
ACTCAGG301.0760978E-647.518-19
TACGTCG1400.047.512-13
CTCAGGC150.00941632647.520-21
CTCAGGA150.00941632647.520-21
CGTCGAC1400.047.514-15
TGTACGT1350.047.510-11
GACTCCC150.00941632647.518-19
Produced by FastQC (version 0.11.5)