FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC046CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC046CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9545
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGACTACGACTGCACGTACAAGGCACCAAACGATCATATGAACAAATG200.20953378732320588No Hit
CGAGACTACGACTGCACGTACACGAAGAAATAAGACCCTTCATTACTCAT200.20953378732320588No Hit
CGAGACTACGACTGCACGTACAATGAACTGGCGACAGTTGAATAGATCGC190.19905709795704557No Hit
CGAGACTACGACTGCACGTACAATGACCGAAATTTCTGGAGGGGTGAAAA160.1676270298585647No Hit
CGAGACTACGACTGCACGTACAATGCCAAGGAAATTGGAAACGGCTGCTT140.1466736511262441No Hit
CGAGACTACGACTGCACGTACACTTCTCAATAAAGCAAGATATCGTAGGA140.1466736511262441No Hit
CGAGACTACGACTGCACGTACAAGAAGCTCAAAAGAGAAATAACGTTCCA130.1361969617600838No Hit
CGAGACTACGACTGCACGTACACATCCAGAAACTGATTGCCCCCAGGGAG120.12572027239392353No Hit
CGAGACTACGACTGCACGTACAGAAAGCACCATCCTCTCTATTGTTATGC120.12572027239392353No Hit
CGAGACTACGACTGCACGTACAACACCATGTCAAGCTTTCAGGTAGACTG120.12572027239392353No Hit
CGAGACTACGACTGCACGTACAGGGGGGAAAATGGACGAAGGACAAGGGT120.12572027239392353No Hit
CGAGACTACGACTGCACGTACAATTAGCACATTAGCTTTCTCTCCTTTTG110.11524358302776323No Hit
CGAGACTACGACTGCACGTACACGCCCCTGGAAGGACAAATCTTCTGGCT110.11524358302776323No Hit
CGAGACTACGACTGCACGTACACAGAGCAGAAATGAAAAGTGGCGAGAGC100.10476689366160294No Hit
CGAGACTACGACTGCACGTACACCGCCCCTGGAAGGACAAATCTTCTGGC100.10476689366160294No Hit
CGAGACTACGACTGCACGTACAAGGCCCTCTTTTCAAACCGTATTTAAAG100.10476689366160294No Hit
CGAGACTACGACTGCACGTACAAGCCCATACACACAAGCAGGCAGGCAGG100.10476689366160294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACT301.1048542E-895.01
GGACTAC450.095.03
ACGAGTG156.4159936E-495.08
AAGACTA301.1048542E-895.02
CGGGACT450.095.01
TACGAGT156.4159936E-495.07
TACGACT8150.095.07
GTGACTA254.2754073E-795.02
GGGACTA450.095.02
CTACGAG156.4159936E-495.06
CTACGAC7950.095.06
CTACGAA254.2754073E-795.06
ACGACTG8200.095.08
CGTGACT254.2754073E-795.01
CGACTGC8100.095.09
GAGACTC156.4159936E-495.02
ACTACGA8200.095.05
GACTACG8200.095.04
AGAGTAC201.6559565E-594.999993
CGAATGC201.6559565E-594.999999
Produced by FastQC (version 0.11.5)