FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC045CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC045CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3626
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTCGTCTCGACGTGTGTGTAAAAGCAGGAGTTTAAAATGAATCCAAA90.2482073910645339No Hit
CGTGTCGTCTCGACGTGTGTGTTTTCATAAGTAATGACGCTTTGATTGCA70.19305019305019305No Hit
CGTGTCGTCTCGACGTGTGTGTCAGCAAAAGCAGGAGTTTAAAATGAATC70.19305019305019305No Hit
CGTGTCGTCTCGACGTGTGTGTTGTTTTCATAAGTAATGACGCTTTGATT70.19305019305019305No Hit
CGTGTCGTCTCGACGTGTGTGTAGTAGCCATTTGTCTGTGAGACCGATGC70.19305019305019305No Hit
CGTGTCGTCTCGACGTGTGTGTTTTTTATCATTAAATAAGCTGAAACGAG60.1654715940430226No Hit
CGTGTCGTCTCGACGTGTGTGTATTGCAATCGTGGACTGGTGTATCTGAA40.11031439602868175No Hit
CGTGTCGTCTCGACGTGTGTGTAGAAGCTTTTTGCTCCAGCATGAGGACA40.11031439602868175No Hit
CGTGTCGTCTCGACGTGTGTGTCTGCAAAAGCAGGAGTTTAAAATGAATC40.11031439602868175No Hit
CGTGTCGTCTCGACGTGTGTGTGTGGGGACATCAAGATACAGCAAGAAGT40.11031439602868175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG3500.089.5583349
GTCTCGA3600.088.3898547
TCTCGAC3550.088.296958
CGTCTCG3450.086.726456
TCGTCTC3500.085.4874955
GTCGTCT3600.084.43214
TGTCGTC3600.084.43213
GTGTCGT3600.081.7935942
CGTGTCG3350.079.071891
TGTGTCG352.265247E-455.0422551
CCGTGTC403.3141987E-947.49305728-29
CTATATA150.00992225647.49305772-73
TTGCAAC150.00992225647.49305782-83
AACCGCA150.00992225647.49305786-87
GGGCAAA150.00992225647.49305726-27
TCGACGG150.00992225647.49305710-11
GTGTAGC150.00992225647.49305718-19
GTGTACG205.0408987E-447.49305718-19
GTTCTGC150.00992225647.49305766-67
CCGCCGT150.00992225647.49305724-25
Produced by FastQC (version 0.11.5)