FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC044CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC044CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGACGATCGACGCGCTGATGAGAGAAGAAATAAATACCTAGAAGA70.27548209366391185No Hit
CGAGTAGACGATCGACGCGCTGAAACGAGAAAGCTCTTATCTCTTGTTCT70.27548209366391185No Hit
CGAGTAGACGATCGACGCGCTGATAACCTATACATAATGTGTCTGCATTT60.23612750885478156No Hit
CGAGTAGACGATCGACGCGCTGATTACATGTTTCAATCTGATTTTGATTC40.15741833923652104No Hit
CGAGTAGACGATCGACGCGCTGATGAGCAAAAGCAGGAGTTTAAAATGAA30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGCATCTGCACTCCCATTCGCTTCTGGTAG30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGCACAGATTGAAAGCGACAGAGAATAGTT30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGAGGAGCTAAGAGAGCAATTGAGCTCAGT30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGAACAGAGGTTATAAGAATGATGGAAAGT30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGAGCAGAAATGAAAAGTGGCGAGAGCAAT30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGACAAAATGACCATCGTCAACATCCACAG30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGAGGGGGGAAAATGGACGAAGGACAAGGG30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGGATTCCTCTTTCAAGATCCATTCCACGA30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGAATAGCATAACAATAGAGAGGATGGTGC30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGCGGCACAGATTGAAAGCGACAGAGAATA30.11806375442739078No Hit
CGAGTAGACGATCGACGCGCTGATGAGCAAAAGCAGGTAGATATTTAAAG30.11806375442739078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGACG2300.095.04
AAGTAGA155.865875E-495.02
AGTAGAC2200.095.03
CAGTAGA155.865875E-495.02
TAGTAGA155.865875E-495.02
GAGTAGA1750.095.02
AGACGAT2350.095.06
GACGATC2300.095.07
CGAGTAG1550.095.01
ACGATCG2250.095.08
TGAGTAG155.865875E-495.01
TAGACGA2350.095.05
CAAGTAG155.865875E-495.01
CGATCGA2250.095.09
CCAGTAG200.00183960671.251
CTAGTAG200.00183960671.251
CGCTGAA204.886964E-447.50000418-19
AGCAGGG204.886964E-447.50000432-33
GCGCTGT252.4409333E-547.50000416-17
GCTGAGG204.886964E-447.50000418-19
Produced by FastQC (version 0.11.5)