FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC035CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC035CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3654
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGTGTTTTT220.60207991242474No Hit
CGAGATGACGAGACGCACGACGCAAAGAGGGCCTATTATCTTTTGCCTAG130.35577449370552816No Hit
CGAGATGACGAGACGCACGACGAAGCGAATGGGAGTGCAGATGCAGCGAT80.21893814997263275No Hit
CGAGATGACGAGACGCACGACGAATCCAGAAAGCACCATCCTCTCTATTG70.19157088122605362No Hit
CGAGATGACGAGACGCACGACGCTTAGAATGACAATTGCATCTGTACCTA70.19157088122605362No Hit
CGAGATGACGAGACGCACGACGCTTAGAATGACAATCGCACGCAAAGAGG70.19157088122605362No Hit
CGAGATGACGAGACGCACGACGATAAGACAGGCAGTTGTGGTCCAGTATC60.16420361247947454No Hit
CGGGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGTGTTTTT60.16420361247947454No Hit
CGAGATGACGAGACGCACGACGAAGAGCATCCCAGTGCTGGGAAGGACCC60.16420361247947454No Hit
CGAGATGACGAGACGCACGACGATCATATGAACAAATGGAGACTGGTGGG60.16420361247947454No Hit
CGAGATGACGAGACGCACGACGATTCAAATGTGAAGAACTTGTATGAAAA50.13683634373289547No Hit
CGAGATGACGAGACGCACGACGCAGAGCAGAAATGAAAAGTGGCGAGAGC50.13683634373289547No Hit
CGAGATGACGAGACGCACGACGCAGAAATGAAAAGTGGCGAGAGCAATTG50.13683634373289547No Hit
CGAGATGACGAGACGCACGACGAGGGACAGAAATTTGAGGAAATAAGGTG50.13683634373289547No Hit
CGAGATGACGAGACGCACGACGGCAAAGGCTATGGAACAGATGGCTGGAT50.13683634373289547No Hit
CGAGATGACGAGACGCACGACGGTGAGCGTGAACACAAATCCTAAAATTC40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGGAAAAGGCAATGGAACAGATGGCTGGAT40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGAACAGAGGTTATAAGAATGATGGAAAGT40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGAATAGCATAACAATAGAGAGGATGGTGC40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGACCAGAGCAGAAATGAAAAGTGGCGAGA40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGATCACATGAACAAATGGAGACTGGTGGG40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGCTTCGCTTCTGGTAGGCCTGCAAATTTT40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGCGTGATCAGTAGAAACAAGGGGGTTTTT40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGAAGGGCAGAAATGAAAAGTGGCGAGAGC40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGGGAGGGGGAAAATGGACGAAGGACAAGG40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGATCAGCAAAAGCAGGTAGATATTTAAAG40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGGGGGACAGAAATTTGAGGAAATAAGGTG40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGAAGAGCTTCCCAGCACTGGGATGCTCTT40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGGGGACAGAAATTTGAGGAAATAAGGTGG40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGCTTCGCGTTACCTTTCTGACATGACCCT40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGCAAAGGCTATGGAACAGATGGCTGGATC40.10946907498631638No Hit
CGAGATGACGAGACGCACGACGGGGGGCAGAAATTTGAGGAAATAAGGTG40.10946907498631638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGATGA2850.095.000012
TGATGAC156.09751E-495.03
GGATGAC156.09751E-495.03
GTGATGA156.09751E-495.02
GGGATGA156.09751E-495.02
CGTGATG156.09751E-495.01
CAAGATG201.5204621E-594.999991
ATGACGA3650.093.698625
GATGACG3550.093.6619644
CGAGACG3550.093.6619649
AGATGAC3250.093.538473
TGACGAG3700.092.4324346
CGAGATG2900.091.724141
ACGAGAC3600.091.0416648
GACGAGA3800.090.07
AAGATGA254.6023404E-576.02
CGGGATG200.00191184771.251
CCAATCT150.00992209347.594-95
ACGCCCA150.00992209347.520-21
CGGAGGG150.00992209347.520-21
Produced by FastQC (version 0.11.5)