FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC026CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC026CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13570
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTCTAGATACTCTGTAGAGCCCCGATTGGGCCAAAAGAACCAAAACC1130.8327192336035372No Hit
CGCGTCTAGATACTCTGTAGAGAAAAACCAAAGTTTAATGATATGGGTCC770.5674281503316139No Hit
CGCGTCTAGATACTCTGTAGAGAAAAGCGAAAGCATAGAAAGTGAGTGTG670.4937361827560796No Hit
CGCGTCTAGATACTCTGTAGAGCCAAAACCAAAGGCCTTAATCCCATTAT280.20633750921149593No Hit
CGCGTCTAGATACTCTGTAGAGGGGGGGAGAAGCTTTCAGTCTCGTACCA270.1989683124539425No Hit
CGCGTCTAGATACTCTGTAGAGATCAGCAAAAGCAGGGCATCGTGGCCAA240.17686072218128224No Hit
CGCGTCTAGATACTCTGTAGAGGGGCCCACCCCTCTCCTCTTTCTCTTCC220.16212232866617537No Hit
CGCGTCTAGATACTCTGTAGAGGCCAAAAGAACCAAAACCAAAGGCCTTA210.15475313190862197No Hit
CGCGTCTAGATACTCTGTAGAGGGGGCCAAAAGAACCAAAACCAAAGGCC180.13264554163596168No Hit
CGCGTCTAGATACTCTGTAGAGAAGAACCAAAGTTTAATGATATGGGTCC160.11790714812085483No Hit
CGCGTCTAGATACTCTGTAGAGGGGGGGCCAAGTGGGAGGTGTTTGGATC160.11790714812085483No Hit
CGCGTCTAGATACTCTGTAGAGATCAGCAAAAGCAGGTGGCACGATGAAA150.1105379513633014No Hit
CGCGTCTAGATACTCTGTAGAGAAAAGCCAAAGTTTAATGATATGGGTCC140.10316875460574797No Hit
CGCGTCTAGATACTCTGTAGAGCTACCAAATGTTCGCGAATCATTGGGAA140.10316875460574797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCTA201.6821108E-595.000012
GATAATC201.6821108E-595.000019
TTTAGAT201.6821108E-595.000015
GCGTTTA201.6821108E-595.000012
GTCTAGG201.6821108E-595.000014
GTCTAGC201.6821108E-595.000014
CGGCTAG201.6821108E-595.000013
AGGTACT201.6821108E-595.000018
GGTACTC201.6821108E-595.000019
CGCGTTT201.6821108E-595.000011
TAGATAA201.6821108E-595.000017
TTAGATA201.6821108E-595.000016
TAGGTAC201.6821108E-595.000017
CGATACT156.476459E-495.08
GATACTT156.476459E-495.09
TCGATAC156.476459E-495.07
GCTAGAT156.476459E-495.05
CGCCTAG156.476459E-495.03
TAGCTAC254.3773616E-795.07
TCTCGAT156.476459E-495.05
Produced by FastQC (version 0.11.5)