FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC025CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC025CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52714
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT9241.7528550290245475No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT7161.3582729445688053No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGGTGACAAA2720.5159919565959706No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGGGTTTT2430.46097810828242974No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGAGTTTAAA2070.3926850552035513No Hit
CGTCTATCATACGACTGTCTACAAAAGCAGGAGTTTAAAATGAATCCAAA1970.37371476268164056No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGTAGATATT1830.34715635315096555No Hit
CGTCTATCATACGACTGTCTACATTCCCATTTAGGGCATTTTGGATAAAG1650.3130098266115264No Hit
CGTCTATCATACGACTGTCTACCAGAGCAGAAATGAAAAGTGGCGAGAGC1640.3111127973593353No Hit
CGTCTATCATACGACTGTCTACATGCGAACAGAGGTTATAAGAATGATGG1620.30731873885495314No Hit
CGTCTATCATACGACTGTCTACAAAAGCAGGTAGATATTTAAAGATGAGT1600.303524680350571No Hit
CGTCTATCATACGACTGTCTACCCGAGAGCAATTGGGACAGAAATTTGAG1170.22195242250635502No Hit
CGTCTATCATACGACTGTCTACCCCTCTTAGTTTGCATAGTTTCCCGTTA930.1764237204537694No Hit
CGTCTATCATACGACTGTCTACCCAAAATACTCAGAGGAAGCAAAATTAA900.17073263269719618No Hit
CGTCTATCATACGACTGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGT820.15555639867966764No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGTCAAATAT800.1517623401752855No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGGTAGATAA750.14227719391433016No Hit
CGTCTATCATACGACTGTCTACGCGGGATCAGTAGAAACAAGGGTGTTTT740.1403801646621391No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGAGTTTTT730.13848313540994803No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGGGAAAACA700.13279204765337482No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTATTTT700.13279204765337482No Hit
CGTCTATCATACGACTGTCTACAAGAGAGCAATTGGGACAGAAATTTGAG670.12710095989680162No Hit
CGTCTATCATACGACTGTCTACCGGGGCAGAAATGAAAAGTGGCGAGAGC660.12520393064461055No Hit
CGTCTATCATACGACTGTCTACATGCAATGAGAACTATTGGGACTCATCC630.11951284288803735No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGCGTTTT630.11951284288803735No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGAGTTTT610.1157187843836552No Hit
CGTCTATCATACGACTGTCTACGCGGGATCAGTAGAAACAAGGTAGTTTT590.11192472587927306No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTCGTTTT590.11192472587927306No Hit
CGTCTATCATACGACTGTCTACATCCAAATGTGCACTGAACTCAAACTCA570.10813066737489092No Hit
CGTCTATCATACGACTGTCTACAAAAGCAGGGTGACAAAAACATAATGGA560.10623363812269984No Hit
CGTCTATCATACGACTGTCTACCAGTACCAATGAACTGGCGACAGTTGAA550.10433660887050877No Hit
CGTCTATCATACGACTGTCTACCAGGGGCGAATGGGAGTGCAGATGCAGC530.10054255036612665No Hit
CGTCTATCATACGACTGTCTACAAGATCCCAATGATATTTGCTGCAATGA530.10054255036612665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAC254.5593515E-795.000019
TGTATCA409.094947E-1295.000013
TTATACG409.094947E-1295.000017
CATAGGA254.5593515E-795.000018
TCATGCG254.5593515E-795.000017
GTATCAT409.094947E-1295.000014
TCATCCG201.728365E-595.000017
CGTGTAT409.094947E-1295.000011
TCATAGG254.5593515E-795.000017
TATAATA201.728365E-595.000015
CTATGAT301.2090823E-895.04
GATACGA301.2090823E-895.08
ATCTATC156.582637E-495.02
CGTATAT156.582637E-495.01
CTAACAT156.582637E-495.04
GTATATC156.582637E-495.02
ATACGAA353.2196112E-1095.09
TCTATGA301.2090823E-895.03
ATACGAC49400.092.788479
CATACGA49650.092.703938
Produced by FastQC (version 0.11.5)