FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC019CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC019CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33017
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTATACGTACGTCTCAGTCCAACACCTTTTTAGATCCAAAAAGGTGT860.260471878123391No Hit
CGAGTATACGTACGTCTCAGTCAGGCCAAACCTAACAAGGCTAGTCTTAC540.16355210951933852No Hit
CGAGTATACGTACGTCTCAGTCTTTATTTTACTTTGGCAGATTCTGGATT530.1605233667504619No Hit
CGAGTATACGTACGTCTCAGTCACCACCAAGGTCATGGTGCTAAGCCATT400.12114971075506556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT500.095.285711
CAAGTAT700.095.285711
CTAGTAT156.482591E-495.285711
CGAGGAT650.095.285711
CGAATAT156.482591E-495.285711
CGAGTAT28550.095.118841
GATACGT650.094.996975
CGTATGT353.1650416E-1094.996979
CGTGCGT201.7190097E-594.996979
GGATACG650.094.996974
ATGTACG550.094.996978
CGTAGGT201.7190097E-594.996979
ACGGACG353.1650416E-1094.996978
ACGTGCG201.7190097E-594.996978
GTATATG550.094.996974
GTATAAG201.7190097E-594.996974
ACGTATG353.1650416E-1094.996978
ATACGTG201.7190097E-594.996976
TATACGG353.1650416E-1094.996975
TACGTAG201.7190097E-594.996977
Produced by FastQC (version 0.11.5)