FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l02_n01_BC009CG_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l02_n01_BC009CG_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11113
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGCTCTATACGTGTAGTCTCAGTCCCATTTTTACCACAAATGCGATAA920.8278592639251326No Hit
CGAGCTCTATACGTGTAGTCTCCCAGGGAGACTACCAGTACCAATGAACT650.5849005669036264No Hit
CGAGCTCTATACGTGTAGTCTCAACCGATTAGAGACCTTGATACTACTAA350.3149464591019527No Hit
CGAGCTCTATACGTGTAGTCTCAGAGGAAGCAAAATTAAACAGAGAAGAA340.3059479888418969No Hit
CGAGCTCTATACGTGTAGTCTCCAGGGAGACTACCAGTACCAATGAACTG310.2789525780617295No Hit
CGAGCTCTATACGTGTAGTCTCCTAACCGAGGTCGAAACGTACGTTCTTT250.22496175650139477No Hit
CGAGCTCTATACGTGTAGTCTCCCACTCTCATCACAGTTTCTCCAAGCGA240.21596328624133898No Hit
CGAGCTCTATACGTGTAGTCTCCAGCCAGCAATGTTACATTTACCCAAAT230.20696481598128316No Hit
CGAGCTCTATACGTGTAGTCTCGAGGAAGCAAAATTAAACAGAGAAGAAA210.1889678754611716No Hit
CGAGCTCTATACGTGTAGTCTCAGCCGATTAGAGACCTTGATACTACTAA210.1889678754611716No Hit
CGAGCTCTATACGTGTAGTCTCCGAAGAAATAAGACCCTTCATTACTCAT210.1889678754611716No Hit
CGAGCTCTATACGTGTAGTCTCCCATTCTCATCACAGTTTCTCCAAGCGA190.17097093494106003No Hit
CGAGCTCTATACGTGTAGTCTCAAGGAAGCAAAATTAAACAGAGAAGAAA190.17097093494106003No Hit
CGAGCTCTATACGTGTAGTCTCAGCAAAAGCAGGTAGATATTTAAAGATG180.16197246468100424No Hit
CGAGCTCTATACGTGTAGTCTCATCACAGTTTCTCCAAGCGAATCTCTGT170.15297399442094844No Hit
CGAGCTCTATACGTGTAGTCTCAAAAGCAGGGTAGATAATCACTCAATGA160.14397552416089265No Hit
CGAGCTCTATACGTGTAGTCTCAACCGCTTAGAGACCTTGATACTACTAA160.14397552416089265No Hit
CGAGCTCTATACGTGTAGTCTCAGGCCCCCTCAAAGCCGAGATCGCGCAG160.14397552416089265No Hit
CGAGCTCTATACGTGTAGTCTCCCAATCTCATCACAGTTTCTCCAAGCGA160.14397552416089265No Hit
CGAGCTCTATACGTGTAGTCTCAGTCCCAATTTTACCACAAATGCGATAA150.13497705390083686No Hit
CGAGCTCTATACGTGTAGTCTCATGCCCATTTAGGGCATTTTGGATAAAG140.12597858364078107No Hit
CGAGCTCTATACGTGTAGTCTCAGGAGATTTCATCAATTATGAGGAGCTA140.12597858364078107No Hit
CGAGCTCTATACGTGTAGTCTCAACCGAGGTCGAAACGTACGTTCTTTCT140.12597858364078107No Hit
CGAGCTCTATACGTGTAGTCTCAAAAGCAGGGGAAAACAAAAGCAACAAA140.12597858364078107No Hit
CGAGCTCTATACGTGTAGTCTCCTCCGATGAGGACCCCAACTGCATTTTT140.12597858364078107No Hit
CGAGCTCTATACGTGTAGTCTCCATACCCATTTAGGGCATTTTGGATAAA130.11698011338072528No Hit
CGAGCTCTATACGTGTAGTCTCAAAAGCAGGTAGATATTTAAAGATGAGT130.11698011338072528No Hit
CGAGCTCTATACGTGTAGTCTCGGGGAAGCAAAATTAAACAGAGAAGAAA130.11698011338072528No Hit
CGAGCTCTATACGTGTAGTCTCAGGGAAGCAAAATTAAACAGAGAAGAAA120.10798164312066949No Hit
CGAGCTCTATACGTGTAGTCTCGGGAAGCAAAATTAAACAGAGAAGAAAT120.10798164312066949No Hit
CGAGCTCTATACGTGTAGTCTCAGGCGCTCCTTCCGTAGAAGGCCCTCTT120.10798164312066949No Hit
CGAGCTCTATACGTGTAGTCTCGGCAGAGGAAGCAAAATTAAACAGAGAA120.10798164312066949No Hit
CGAGCTCTATACGTGTAGTCTCGGCAGGGGAAAACAAAAGCAACAAAAAT120.10798164312066949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGG254.324338E-795.09
TATACGG201.6685346E-594.999998
CGCTATA201.6685346E-594.999995
GCTATAC201.6685346E-594.999996
TATCCGT156.4451125E-494.999998
GCGCTAT201.6685346E-594.999994
AGCGCTA201.6685346E-594.999993
ATCCGTG156.4451125E-494.999999
CTATCCG156.4451125E-494.999997
TATACGT9850.093.55338
AGCTCTA10450.092.727273
GAGCTCT10000.092.625012
ATACGTG9800.092.576549
CTATACG10450.092.272737
TCTATAC10250.092.219526
CGAGCTC10000.092.150011
GCTCTAT10550.091.848344
CTCTATA10450.091.818185
AAGCTCT353.2734533E-881.428572
CAAGCTC353.2734533E-881.428571
Produced by FastQC (version 0.11.5)