FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6937238
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGTCTACTACTCTACTGTTCCTCTCGATATTCTTCCCTCATAGACT150940.21757938822338224No Hit
CGCTACACGCGTCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA109480.15781496901216305Illumina Single End PCR Primer 1 (100% over 36bp)
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT105390.15191925086035682No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT101120.14576406345003587No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC96350.13888812809939632Illumina Single End PCR Primer 1 (100% over 50bp)
CGTGTCGCGCACTCGACTCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG69800.10061641246847809Illumina Single End PCR Primer 1 (100% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCG223550.092.705834
CGTGTAT239350.092.6581951
GCGCGTC194900.092.636585
CGACTAT274300.092.263891
CGCGTCA195600.091.989376
CGCGCGT196950.091.768824
CGTCGTA144300.091.70821
TCGCGCG199300.091.663933
CGAGATG152950.091.459581
CGTATAG253550.091.459231
TGTCGCG209150.091.389563
GCGCACT207900.091.345017
TCACGTC226850.091.23163
TCGTGTG223150.090.913678
CGCGCAC209300.090.870186
CGCGTCT49100.090.8400651
CGTGTGC222650.090.776499
CGAGTAG138750.090.6179661
CATACGA198500.090.549738
GACTATA278550.090.532172
Produced by FastQC (version 0.11.5)