FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC379CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC379CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164605
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT37092.253272986847301No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGGGTTTT31021.884511406093375No Hit
AGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTTTT15210.9240302542450108No Hit
GAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTTT12340.7496734607089699No Hit
GACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTA9550.5801767868533763No Hit
CGAGACGATGTGTGATTCACCCAGGTGCTGTTTTCATATGTGATGATTCT5110.3104401445885605No Hit
TGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT4430.26912912730475985No Hit
CGAGACGATGTGGCCATTTCTTCGCCGGTGAATGAGAAAATGTGAATGTG4390.2666990674645363No Hit
CGAGACGATGTGTCCTTCCAGGGGCGGGGAGTCTTTGAGCTCTCGGACGA4140.25151119346313905No Hit
CGTCACGTCGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT4070.2472585887427478No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTATTTT3460.2102001761793384No Hit
ACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTAT3030.18407703289693508No Hit
CGAGACGATGTGTACGTACTACCCGGGAACAACTAGGTCAGAAGTTTGAA2980.18103945809665561No Hit
CGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATC2620.15916891953464354No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGTAGTTTT2540.1543087998541964No Hit
CGAGACGATGTTCAGACCGAGAGTGCTGCCTCTTCCCTTTAGAGACTTCT2460.14944868017374927No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTGTTTTTTATCACT2420.14701862033352572No Hit
CGAGACGATGTGTACGTACTACCCGCGCTGGGATGTTCCTCTAGATATTT2300.13972844081285501No Hit
CGAGACGATGTGTCCATACACACAGGCAGGCAGGCAAGACTTGTGAGCAA2010.12211050697123417No Hit
AGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTATTTTTC2000.12150299201117828No Hit
CGAGACGATGTGTACGTACTACGCGGGATCAGTAGAAACAAGGGGGTTTT1890.11482032745056348No Hit
CGAGACGATGTGTACGTTCTGGAAAGGAAGACTGCTGTTTATAGCTCCCT1870.11360529753045169No Hit
GATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCA1650.10023996840922207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATG201.739374E-595.000014
ATAGTCG201.739374E-595.000013
GCGTCGA201.739374E-595.000019
CTGTGTA201.739374E-595.000018
AGTCGTA201.739374E-595.000015
ACGCGTC201.739374E-595.000017
CTAGACG301.2254532E-895.01
ACTATAC301.2254532E-895.03
CGAGTAG301.2254532E-895.01
GCTATCA1100.095.02
GTCTACT900.095.06
CGCTATC1100.095.01
CGCTACA254.602971E-795.01
GTGTCGC1550.095.02
CGAGCCG156.6077686E-495.01
ACGTCAC254.602971E-795.08
CGTGTCG2150.095.01
CGATGTA500.095.06
CGAGACC353.274181E-1095.01
CGATGCG353.274181E-1095.06
Produced by FastQC (version 0.11.5)