FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC378CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC378CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGACGCACGTGTCGATACTAGATACGCGTGATCAGTAGAAACAAGGGT214.285714285714285No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTGTTTTTTATCACT17.142857142857142No Hit
CGAGACGATGTTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTT17.142857142857142No Hit
CGAGACGATGTGTACGTACTACCCGGGAACAACTAGGTCAGAAGTTTGAA17.142857142857142No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGCGAAAGCAGGGTGTTTTT17.142857142857142No Hit
CGCGCGATACACGTGACGACGCCGACAATTGATCCCTCTTTGTATCAGAA17.142857142857142No Hit
CGACTATACGTCACGTGCTGTATGGCGGGAACAACTAGGTCAGAAGTTTG17.142857142857142No Hit
CGTGTCGCGCACTCGACTCGACCGGCAGAGAATAGTTTTGAGCAAATAAC17.142857142857142No Hit
CGAGACGATGTGTACGTACTACGGAAGGAGTACCAGAGTCTATGAGGGAA17.142857142857142No Hit
AAGACGACAGAGAATAGTTTTGAGCAAATAACATTTATGCAAGCATTACA17.142857142857142No Hit
CGTATGAGTACGATATATACTGCAACACACAAACGAAAACGTTCTAATCT17.142857142857142No Hit
CGAGACGATGTGTCGATACTAGAGAGGCGGGAAAAACAAGGGCAGGAGGT17.142857142857142No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT17.142857142857142No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)