FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC351CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC351CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6758
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTTTGAGGGGGCCTGACGGGATGATAGAGAGTACGTACGTTTCGACC400.5918910920390648No Hit
GTGCCTCATCGGATTCTTCTTTCAGAATCCGCTCCACTATCTGCTTTCCA260.38472920982539216No Hit
ATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCGCTCCACTATCTG210.31074282332050907No Hit
AGTGCCTCATCGGATTCTTCTTTCAGAATCCGCTCCACTATCTGCTTTCC150.2219591595146493No Hit
CCTCATCGGATTCTTCTTTCAGAATCCGCTCCACTATCTGCTTTCCAGCA140.20716188221367265No Hit
GTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCGCTCCACTAT120.17756732761171945No Hit
CCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCGCTCC120.17756732761171945No Hit
GCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCGCTC120.17756732761171945No Hit
CGGCGAAGCCGATCAAGGAATGGGGCATCACCTAGTTCTTGGTCTGCAAC110.16277005031074282No Hit
GGATTCTTCTTTCAGAATCCGCTCCACTATCTGCTTTCCAGCACGTGTGG110.16277005031074282No Hit
GATTCTTCTTTCAGAATCCGCTCCACTATCTGCTTTCCAGCACGTGTGGC110.16277005031074282No Hit
GAGGCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCG100.1479727730097662No Hit
CGCGCGATCCCCGTTCCCATTAAGGGCATTTTGGACAAAGCGTCTACGCT90.1331754957087896No Hit
AAGCCGATCAAGGAATGGGGCATCACCTAGTTCTTGGTCTGCAACTCGTT90.1331754957087896No Hit
CAGAGGCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATC90.1331754957087896No Hit
ATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCGCTCCAC90.1331754957087896No Hit
CATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCGCTCCA90.1331754957087896No Hit
TCTTCTTTCAGAATCCGCTCCACTATCTGCTTTCCAGCACGTGTGGCTGT80.11837821840781297No Hit
CGGATTCTTCTTTCAGAATCCGCTCCACTATCTGCTTTCCAGCACGTGTG80.11837821840781297No Hit
GTGCTGCCCCTTCCTCTTAGGGATTTCTGATCTCGGCGAAGCCGATCAAG80.11837821840781297No Hit
GGCCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCGCT70.10358094110683633No Hit
AGGGTGCTGCCCCTTCCTCTTAGGGATTTCTGATCTCGGCGAAGCCGATC70.10358094110683633No Hit
CTCCACTATCTGCTTTCCAGCACGTGTGGCTGTCTCGATGTCCAGACCGA70.10358094110683633No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)