FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC348CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC348CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145847
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGAGACACTATTTGCTTTCCAACACAAGTAGCTGTTTCGATGTTCAG6740.4621281205647014No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAGAGAACGTACGTTTCGACC5410.37093666650668167No Hit
CGTCCCTCTATTATCTCAAATCTGTGCTTTAAAAGTGCATTTGGGTCCTC3830.2626039616858763No Hit
CTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACAAATCCTAAAA2410.16524165735325375No Hit
CGTCGAGACACTAAATAAGCTGAAACGAAAACGTTCTAATCTCTTGTTCC1850.12684525564461388No Hit
GCTGGACCAATTGGGTTAAGTTGCGGTGCTCCAGTTTCGGTATTTTTTGT1730.11861745527847675No Hit
CGTCGAGACACTCCTGGGTCCCCAAAAGAAATCGATCCATTTTGAACACG1730.11861745527847675No Hit
CGTCGAGACACTACAACACAGAAACGGAAAATGGCGGGAACAACTAGGTC1700.11656050518694248No Hit
CGTCGAGACACTCCATGAGAGCCTCAAGATCGGTATTCTTTCCAGCAAAT1590.10901835485131678No Hit
CGTCGAGACACTACAACACAGAAACGGAAAATGGCGGGAACAACTAGGGC1580.10833270482080537No Hit
CGTCGAGACACTACAGAGACATAAGCATTTTCTGTATGATAGAGGGCCTT1540.10559010469875967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC450.095.000013
CGAGTAG450.095.000011
ATCGTCT1050.095.000016
CGTGTAT3600.095.000011
GACTATA450.095.000012
TCGCATA254.6003152E-795.08
CTCACGC254.6003152E-795.08
GTCGATA254.6003152E-795.02
CGTATAT301.2243618E-895.04
CGTATAG1100.095.01
AGATATA301.2243618E-895.08
CTATCAC600.095.03
CTGACGT254.6003152E-795.03
TGTGATA301.2243618E-895.05
GAGACCC156.6062406E-495.05
CTACGAC254.6003152E-795.06
TCGTATA301.2243618E-895.03
CGTCGTA550.095.01
GTGAGCT156.6062406E-495.02
CGAGATG156.6062406E-495.01
Produced by FastQC (version 0.11.5)