FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC346CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC346CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684881
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTATATACTCTAGCGAGTGGCAAGGACAAGACAAATCCTAAAATCCC50670.7398365555476061No Hit
CGTGTATATACTCTAGCGAGTGGCCGAGACAAGACAAATCCTAAAATCCC49590.7240673927295399No Hit
CGTGTATATACTCTAGCGAGTGGCCAAGACAAGACAAATCCTAAAATCCC43840.6401112018000208No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCGAAAGACAAGACAAATCCTAA37930.5538188386011584No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCCAAAGACAAGACAAATCCTAA32220.4704466907389751No Hit
CGTGTATATACTCTAGCGAGTGGCGCAGACAAGACAAATCCTAAAATCCC31940.46635838926762463No Hit
CGTGTATATACTCTAGCGAGTGATTAGCAAAAGCAGGTAGATATTGAAAG30070.4390543758696766No Hit
CGTGTATATACTCTAGCGAGTGGCAAAGGCAAGACAAATCCTAAAATCCC29640.4327759128958169No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCAAAAGACAAGACAAATCCTAA29560.4316078267611454No Hit
CGTGTATATACTCTAGCGAGTGATTAGCGAAAGCAGGTAGATATTGAAAG25630.37422559539540445No Hit
CGTGTATATACTCTAGCGAGTGAACAGCAAAAGCAGGTAGATATTGAAAG22760.3323205053140619No Hit
CGTGTATATACTCTAGCGAGTGGCGAAGACAAGACAAATCCTAAAATCCC22620.3302763545783866No Hit
CGTGTATATACTCTAGCGAGTGGCGACGACAAGACAAATCCTAAAATCCC22400.3270641177080398No Hit
CGTGTATATACTCTAGCGAGTGAACAGCGAAAGCAGGTAGATATTGAAAG22310.32575002080653426No Hit
CGTGTATATACTCTAGCAAGTGCGGAGGCCATGGTCATTTTAAGTGCCTC21810.31844948246483695No Hit
CGTGTATATACTCTAGCGAGTGGCACAGACAAGACAAATCCTAAAATCCC21560.3147992132939883No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAAAAGCAGGTAGATATTGAAAG21030.3070606426517891No Hit
CGTGTATATACTCTAGCGAGTGATCAGCGAAAGCAGGTAGATATTGAAAG19700.2876412106628743No Hit
CGTGTATATACTCTAGCGAGTGAGGCCCCCTCAAAGCCGAGATCGCACAG18020.26311140183477133No Hit
CGTGTATATACTCTAGCGAGTGCGGAGGCCATGGTCATTTTAAGTGCCTC17570.2565409173272437No Hit
CGTGTATATACTCTAGCGAGTGGCGGAGACAAGACAAATCCTAAAATCCC16880.24646617441570143No Hit
CGTGTATATACTCTAGCGAGTGGCCAGGACAAGACAAATCCTAAAATCCC15690.2290908931624618No Hit
CGTGTATATACTCTAGCGAGTGACCAGCGAAAGCAGGTAGATATTGAAAG14940.2181400856499158No Hit
CGTGTATATACTCTAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGG14160.206751245836868No Hit
CGTGTATATACTCTAGCGAGTGGCGAGGACAAGACAAATCCTAAAATCCC13300.19419431988914862No Hit
CGTGTATATACTCTAGCGAGTGGAGGGGCAAAGACAAGACAAATCCTAAA12840.1874778246147871No Hit
CGTGTATATACTCTAGCGAGTGCAGCGAAAGCAGGTAGATATTGAAAGAT12800.1868937815474513No Hit
CGTGTATATACTCTAGCGAGTGATAAGCGAAAGCAGGTAGATATTGAAAG12750.18616372771328157No Hit
CGTGTATATACTCTAGCGAGTGGCGGCGACAAGACAAATCCTAAAATCCC12690.1852876631122779No Hit
CGTGTATATACTCTAGCGTGAACACAAATCCTAAAATCCCCTTAGTCAGA12480.18222143700876503No Hit
CGTGTATATACTCTAGCGAGTGACCAGCAAAAGCAGGTAGATATTGAAAG12390.1809073401072595No Hit
CGTGTATATACTCTAGCGAGTGATAAGCAAAAGCAGGTAGATATTGAAAG12350.18032329703992372No Hit
CGTGTATATACTCTAGCGAGTGGCCCAGACAAGACAAATCCTAAAATCCC12100.17667302786907507No Hit
CGTGTATATACTCTAGCGAGTGAGCAGCAAAAGCAGGTAGATATTGAAAG11480.1676203603253704No Hit
CGTGTATATACTCTAGCGAGTGAGCAGCGAAAGCAGGTAGATATTGAAAG11450.16718232802486857No Hit
CGTGTATATACTCTAGCGAGTGGGGGGGCAAATGCAACAACAAGAGGATC11210.16367806962085385No Hit
CGTGTATATACTCTAGCGAGTGCTCAGCAAAAGCAGGTAGATATTGAAAG10790.15754561741382808No Hit
CCATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCTCTCT10710.15637753127915655No Hit
CGTGTATATACTCTAGCGAGTGGCAGAGACAAGACAAATCCTAAAATCCC10480.15301928364197576No Hit
CGTGTATATACTCTAGCGAGTGATGAGCAAAAGCAGGTAGATATTGAAAG10070.14703284220178398No Hit
CGTGTATATACTCTAGCGAGTGATGAGCGAAAGCAGGTAGATATTGAAAG9810.14323656226410136No Hit
CGTGTATATACTCTAGCGAGTGGCGCCGACAAGACAAATCCTAAAATCCC9560.13958629309325268No Hit
CGTGTATATACTCTAGCGAGTGGCAACGACAAGACAAATCCTAAAATCCC9450.13798017465807927No Hit
CGTGTATATACTCTAGCGAGTGGAGAGGCAAAAGACAAGACAAATCCTAA8770.12805144251337094No Hit
CGTGTATATACTCTAGCGAGTGGCCGGGACAAGACAAATCCTAAAATCCC8690.12688335637869938No Hit
CGTGTATATACTCTAGCGAGTGGAGAGGCGAAAGACAAGACAAATCCTAA8400.12264904414051492No Hit
CGTGTATATACTCTAGCGAGTGGGGGTGCAGATGCAACGATTCAAGTGAT8300.12118893647217546No Hit
CGTGTATATACTCTAGCGAGTGGCAAAGACAAGACAAATCCTAAAATCCC8270.12075090417167363No Hit
CGTGTATATACTCTAGCGAGTGGAAAAGACAAGACAAATCCTAAAATCCC7890.11520249503198367No Hit
CGTGTATATACTCTAGCGAATGGGGGTGCAGATGCAACGATTCAAGTGAT7810.11403440889731209No Hit
CGTGTATATACTCTAGCGAGTGGAGGGCAAAAGACAAGACAAATCCTAAA7790.11374238736364418No Hit
CGTGTATATACTCTAGCGAGTGGGAAAGACAAGACAAATCCTAAAATCCC7780.11359637659681025No Hit
ATGGTCATTTTAAGTGCCTCATCGGATTCTTCTTTCAGAATCCTCTCTAC7500.10950807512545976No Hit
CGTGTATATACTCTAGCGAGTGGAGGGCGAAAGACAAGACAAATCCTAAA7300.10658785978878083No Hit
CGTGTATATACTCTAGCGAGTGATCCGCAAAAGCAGGTAGATATTGAAAG7280.10629583825511293No Hit
CGTGTATATACTCTAGCGAGTGCGGCAACGATTCAAGTGATCCTCTTGTT7210.10527376288727532No Hit
CGTGTATATACTCTAGCGAGTGATCAACAAAAGCAGGTAGATATTGAAAG7200.10512775212044136No Hit
CGTGTATATACTCTAGCGAGTGGGGCAGGGAATGAGTCCCAATGGCTCTC7110.10381365521893585No Hit
CGTGTATATACTCTAGCGAGTGATCCGCGAAAGCAGGTAGATATTGAAAG6960.10162349371642665No Hit
CGTGTATATACTCTAGCGAGTGCTCAGCGAAAGCAGGTAGATATTGAAAG6960.10162349371642665No Hit
CGTGTATATACTCTAGCGAGTGATCAACGAAAGCAGGTAGATATTGAAAG6940.10133147218275874No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATATC156.6167745E-495.06
CGTGTAA600.095.01
CGTCGAT353.2923708E-1095.01
ATACGTG450.095.09
ACGACTT156.6167745E-495.08
CGTGTTA201.7433247E-594.999991
CGCGTCT201.7433247E-594.999991
CGATAGT750.094.999991
CGTGTAT370600.094.6539461
TGTCGCG2800.093.303573
GTGTATA376050.093.231622
CGGGTAT2500.093.1000061
GCGCGTC2400.093.0208365
TGTATAT378900.092.3924563
TATACTC375250.092.202537
GAGACGT15300.091.8954242
ATATACT379650.091.784546
GTGTCGC2850.091.666672
GTATATA384450.091.5899354
CGTGTCG3900.091.346151
Produced by FastQC (version 0.11.5)