FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC345CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC345CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256936
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTGTTTTTTATCACT9750.37947193075318364No Hit
CGAGACGTAGATGCCGGATCAGACCGAGTGATGGTATCACCCCTGGCTGT9230.35923342777968054No Hit
CGAGACGTAGCTGTTTCGATGTTCAGACCGAGAGTGCTGCCTCTTCCCTT7780.30279914064202756No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGGGTTTTTTATCACT5800.2257371485506118No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTCGTTTTTAAATTAT5630.21912071488619733No Hit
CGAGACGATGTGTACGTACTACGCGTGATCAGTAGAAACAAGGGTGTTTT4940.19226577824827973No Hit
CGAGACGTATTTTGACTTGGTTTCTAAAGTGTACAGGGCCAAAGGTTCCT4270.16618924557088147No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGGTATTTTTCATTAAT3930.1529563782420525No Hit
CGATACTCGTCATCTACGTCTCGAGATCGGAAGAGCGTCGTGTAGGGAAA3910.15217797428153315Illumina Single End PCR Primer 1 (100% over 27bp)
CGAGACGTAGATTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCAC3680.14322632873556063No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTAGTTTTTTACTCTA3600.1401127128934832No Hit
AGACGTAGATGACGAGTATCAGTAGAAACAAGGGTGTTTTTTATCACTAA3500.13622069309088644No Hit
CGAGACGTAGATGACAGAGTGTGTCACTGTCACATTCTTTTCAAGTACTG3460.13466388516984773No Hit
CGAGACGTAGATCACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCT3080.11987420991998007No Hit
CGAGACGTAGATGACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCAC3060.11909580595946072No Hit
CGAGACGTAGATGACTGTTCTCAAGCAGGTTGACAGAGTGTGTCACTGTC2720.10586293863063176No Hit
CGAGACGTAGATGGAAAGTGGGTGAGGGAACTCGTCCTTTATGACAAAGA2580.1004141109069963No Hit
GACGTAGATGACGAGTATCAGTAGAAACAAGGGTGTTTTTTATCACTAAA2570.10002490892673663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT201.7412416E-595.000012
ACACGTA450.095.000013
TACTTTA201.7412416E-595.000019
GACGTTG450.095.000014
GACGTAA409.094947E-1295.000014
CGTAGTT201.7412416E-595.000016
GTACGCT409.094947E-1295.000015
CGAACGT156.6120276E-495.01
ACGTTGA500.095.05
GGCGTAG254.6103924E-795.04
CGCGCGT2000.095.04
CGTCTAT1400.095.01
CATAGAT353.2923708E-1095.06
TAGATAG156.6120276E-495.08
CGTGTAT13800.093.967391
TGTCGCG4300.093.8953553
TCACGTC4150.093.855423
CACGTCG4100.093.841464
ACGATGT3500.093.642855
GTGTATA13850.093.28522
Produced by FastQC (version 0.11.5)