FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC337CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC337CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428868
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGAGTAGTACAGTGTCTGAAACGAAAACGTTCTAATCTCTTGTTCCA21610.5038846451588833No Hit
CGTAGAGTAGTACCAGAGTCTATGAGGGAAGAATATCGAGAGGAACAGCA16470.3840342483001763No Hit
CGTAGAGTAGTACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAG8570.1998283854239533No Hit
CGTAGAGTAGTACAGTGTCTGACCGAGAGTGCTGCCTCTTCCCTTTAGAG6250.14573248645270806No Hit
CGTAGAGTAGTACAGTGTCTGAAGAAACAAGGTCGTTTTTAAATTATTCG6020.1403695309512484No Hit
CGTAGAGTAGCTGTTTCGATGTTCAGACCGAGAGTGCTGCCTCTTCCCTT5820.13570609138476172No Hit
CGTAGAGTAGTACAGTGTTGTTTTGACTTCCAGTTTGGATTGAGTGACTA5650.1317421677532481No Hit
CGTAGAGTAGTACAGTGTCTGATGGCCATCCGAATTCTTTTGGTCGCTGT5440.1268455562084371No Hit
CGTAGAGTAGTACAGTGTCTGACCTAGTTGTTCCCGCCATTTTCCGTTTC5360.12498018038184244No Hit
CGTAGAGTAGTACAGTGTCTGACAGCGACCAAATTAATTGATGGCCATCC4750.11075668970405812No Hit
CGTAGAGTAGTACAGTGTCTGAATGAGCGGGTCATTATGGTGAAACATGA4670.10889131387746345No Hit
CGTAGAGTAGTACAGTGTCTGACTTAGCCAACAGTGTCCTGCATACTTTC4620.10772545398584178No Hit
CGTAGAGTAGTACAGTGTCTGACCCAGCTATCTCAATGCATGTGTTAGGA4320.1007302946361118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGC500.095.08
GTCGATA156.6150737E-495.02
GCGCACT3750.095.07
GTAATAC156.6150737E-495.07
GAGACTA450.095.02
GTATCTA156.6150737E-495.05
TCGAGAC1950.095.03
AGTAGGC156.6150737E-495.06
CGACTAA156.6150737E-495.07
ACTCACG500.095.07
ACGAGTC254.6156856E-795.03
AGTACAT600.095.09
TACGTCA850.095.07
TAGCGTA201.7425786E-594.999993
TAGTGCA550.094.999998
GAGACGT7700.094.383122
GTGTCGC3950.093.797472
CACGTCG4000.092.6254
TCACGTC4000.092.6253
TCGCGCA3950.092.594945
Produced by FastQC (version 0.11.5)