FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC333CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC333CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63553
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTTTGAGGGGGCCTGACGGGATGATAGAGAGAACGTACGTTTCGACC1290.20298018976287507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTATA201.7311251E-595.000012
CGTAGTA201.7311251E-595.000011
CGTGTAT409.094947E-1295.000011
TCGAGAC450.095.000013
ATACAGC201.7311251E-595.000013
TGATAGT156.588943E-495.07
ATCTATA156.588943E-495.07
CGTATAG500.095.01
CGAGTGT156.588943E-495.01
GCGCGTC700.095.05
CTATAGT156.588943E-495.09
CGCGCGT700.095.04
CGTCTAT156.588943E-495.01
CGCGATA156.588943E-495.03
TGTGATA156.588943E-495.05
GTCAGTC156.588943E-495.02
ACGATGT550.095.05
GAGACGT1000.095.02
TATCTAT156.588943E-495.06
CGCTATC301.2132659E-895.01
Produced by FastQC (version 0.11.5)