FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC331CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC331CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33469
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGCAAAAGCAGGCCTATCAGAAACGAATGGGGGTGCAGAT4691.4012967223400759No Hit
ACGCGTGATCAGCAAAAGCAGGTCTATCAGAAACGAATGGGGGTGCAGAT4461.3325764139950402No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGA3901.1652574023723445No Hit
CGTACTCTCGCTATTGCCGCAAATATCATTGGGATCTTGCACTTGACATT3561.0636708596014222No Hit
ACGCGTGATCAGTAGAAACAAGGCAGTTTTTTACTCCAGCTCTATGCTGA1390.41530968956347664No Hit
CGTACTCTCGCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTC1150.34360154172517854No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAA950.2838447518599301No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT910.2718933938868804No Hit
CGTACTCTCGCATAGACTACTACAGCTCTATGCTGACAAAATGACCATCG630.18823388807553257No Hit
CGTACGTCTACTACTCTACTGTTCCTCTCGATATTCTTCCCTCATAGACT630.18823388807553257No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGTAGTTT600.17927036959574533No Hit
CGTACTCTCGCATAGACTACTAAGGCCCTCCTTTCAGTCCGTATTTAAAG600.17927036959574533No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGTCTATCA530.15835549314290837No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGTAGAAACAAGGGTGTTT520.15536765364964594No Hit
CGTACTCTCTTATGACAAAAACGTCTCCTTTGGAACCAATTCTTATGCTA410.1225014192237593No Hit
CGTACTCTCGCATAGACTACTACGCGTGATCAGCAAAAGCAGGCCTATCA380.11353790074397205No Hit
CGTACTCTCTCTTGACTGATGATCCGCTTGTTCTCTTAAATGTGAATCCA370.11055006125070962No Hit
GACAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGA350.10457438226418476No Hit
CGTGATCAGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAA350.10457438226418476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGT156.5614074E-495.07
GTGTATA500.095.02
AGTAGAC353.1650416E-1095.03
ACGTACA156.5614074E-495.07
GTACAGC156.5614074E-495.09
GCGTGAC156.5614074E-495.02
AGACGTA156.5614074E-495.03
GCTGTCG156.5614074E-495.09
GACGTAC156.5614074E-495.06
ATACGAC650.095.09
CGTACAG156.5614074E-495.08
GTGACGT156.5614074E-495.04
CGAGTAG353.1650416E-1095.01
CGTGTCG600.095.01
CGTGTAT450.095.01
CGTCGTA156.5614074E-495.01
GTCTACT353.1650416E-1095.06
CGAGATG301.1956217E-895.01
AGACACT254.5226625E-795.06
TGACGTA156.5614074E-495.05
Produced by FastQC (version 0.11.5)