FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC330CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC330CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63124
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGA7531.1928901843989608No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAA4700.7445662505544642No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACC3730.5909004499081174No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT2610.41347189658450034No Hit
ACGCGTGATCAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGC2520.3992142449781383No Hit
CGAGTATATAGTACTGTGTCAACAGTGTCGGTTGAATTGTTCGCATGGTA1840.29148976617451366No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAGACTACTTGTCAAT1660.2629744629617895No Hit
CGAGTATATAGTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATTATT1660.2629744629617895No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACAC1470.23287497623724732No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCCTCATATTTCTGAAATTC1300.20594385653634117No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT1290.20435967302452315No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAAAAGCAGGTAGATATTGAAAG1250.19802293897725112No Hit
GTGCTGCCCCTTCCTCTTAGGGATTTCTGATCTCGGCGAAGCCGATCAAG1210.1916862049299791No Hit
CGAGTATATAGTACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCCA1110.175844369811799No Hit
ACGCGTGATCAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCA960.15208161713452886No Hit
ACGCGTGATCAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAAT940.14891325011089285No Hit
CGAGTATATAGTACGCGTGATCAGCAAAAGCAGGGGAAAATAAAAACAAC930.14732906659907483No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGTAGTTTT870.13782396552816678No Hit
CGAGTATATAGTACTACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACT850.13465559850453077No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCTTTAATTGTCGTACTCCT770.12198213040998669No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGTAGATATT720.11406121285089665No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTAGTTTTTTACTCCA710.11247702933907865No Hit
CGAGTATATAGTACTGTCTCTGCACACACACATCACTTTGCCGGTATCAG690.10930866231544262No Hit
ACGCGTGATCAGCAAAAGCAGGCCTATCAGAAACGAATGGGGGTGCAGAT660.1045561117799886No Hit
CGAGTATATAGTACTGTCACTACAGAGACATAAGCATTTTCATTCTGATA650.10297192826817059No Hit
CGAGTATATAGTACTGACGCGTGATCAGTAGAAACAAGGGTGTTTTTTAT640.10138774475635258No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGGTGTTTT640.10138774475635258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT1700.095.000011
GAGGATA500.095.02
ACTATAC156.5887236E-495.03
CTACTAG301.213084E-895.09
GAGCTAC301.213084E-895.04
GCGCGTC1200.095.05
CTATCAC301.213084E-895.03
GCTATCA301.213084E-895.02
CGCGCAC2150.095.06
ATTGTAC156.5887236E-495.08
TATATAT156.5887236E-495.05
GTCAGTC254.5699016E-795.02
ACGATGT353.237801E-1095.05
CTACGTC301.213084E-895.07
TACGATA301.213084E-895.09
CGCTATC301.213084E-895.01
CGCTACA156.5887236E-495.01
GTGTCGT700.095.02
ACGTCAC156.5887236E-495.08
CGTGTGC1300.095.09
Produced by FastQC (version 0.11.5)