FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC324CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC324CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91721
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA3640.3968556819049072No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC3090.3368912244742207No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC2920.3183567558138267No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAAT1970.2147817838880954No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT1750.1907960009158208No Hit
CGTCAGTCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATT1690.1842544237415641No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA1670.18207389801681184No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT1510.16462969221879395No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT1310.14282443497127156No Hit
CGTCAGTCTCTGCGCGATCTCGGCTTTGAGGGGGCCTGACGGGATGATAG1220.1330120692098865No Hit
ACGCGTGATCAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGC1120.1221094405861253No Hit
ACGCGTGATCAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGA1110.1210191777237492No Hit
ACGCGTGATCAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAAT1020.11120681196236412No Hit
GGTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAA940.10248470906335519No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT940.10248470906335519No Hit
CGTCAGTCTCTATACGTCTAGCCGGCGCCATTCTGTTTTCATGCCTGATT920.10030418333860293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAC201.7352468E-595.000014
CGACAGC201.7352468E-595.000014
CGTCGTA800.095.000011
ATAGTCG201.7352468E-595.000013
GACGACA201.7352468E-595.000012
ACATACA201.7352468E-595.000013
CGATAGT201.7352468E-595.000011
AGTACGA201.7352468E-595.000017
GTACAGC254.5866E-795.09
CGAGTAG1000.095.01
GCGCGTC301.2192686E-895.05
CTATCAC500.095.03
CTATAGT156.598353E-495.09
CGTGACG254.5866E-795.03
GAGTACA450.095.04
CTGACGT254.5866E-795.03
TGTGATA254.5866E-795.05
AGAGTAC450.095.03
ACGATGT156.598353E-495.05
GAGACGT450.095.02
Produced by FastQC (version 0.11.5)