FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC320CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC320CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31880
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTAGTCGCGCTTCAATCGCTGGCCCAGCAGCCATGCCTTCTAAGCAGT1250.39209535759096614No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT1200.37641154328732745No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA950.2979924717691343No Hit
ACGCGTGATCAGCAAAAGCAGGAATCACTCTAGAATTATGCCTTTGACAT610.19134253450439148No Hit
ACGCGTGATCAGTAGAAACAAGGCCGTTTTTAAACAATTCGACACTAATT600.18820577164366373No Hit
CGCTAGTCGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACA580.18193224592220827No Hit
ACGCGTGATCAGCAAAAGCAGGCCGGACTCATAAACTGGGGCGATAACAG510.15997490589711416No Hit
CGCTAGTCGCGTTGGGGCAAGAAGGGGATTGAACTCAGCAATGAGATGGA430.13488080301129235No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT390.12233375156838143No Hit
ACGCGTGATCAGTAGAAACAAGGTCTGAGGAAGCAAGGCCCATAGTTGAC350.10978670012547051No Hit
CGCTCGTTGCCTCCTTCATCTACCTGCTGGTCTCGGGGAACTCGCTGAAC340.1066499372647428No Hit
GGATTGAACTCAGCAATGAGATGGAAGTATGGAACTTAGAAGCTTACTAC330.10351317440401506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGAGT156.55849E-495.07
GAGCTCT156.55849E-495.02
CGCTAGT2250.095.01
GAGTCGC156.55849E-495.05
GAGTATA500.095.02
ATACGTG156.55849E-495.09
AGAGTAC353.1650416E-1095.03
GCTCGTT156.55849E-495.02
GACGACG156.55849E-495.02
CGAGTCG156.55849E-495.04
GTCTATC550.095.02
GAGAGTA353.1650416E-1095.02
CGTGAGC156.55849E-495.01
TGAGCTA156.55849E-495.03
CATACGA500.095.08
CTCGTTG156.55849E-495.03
TAGACGA156.55849E-495.05
CGACATA156.55849E-495.01
GCGTGCA201.71781E-594.999999
TCTCGAC201.71781E-594.999998
Produced by FastQC (version 0.11.5)