FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC317CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC317CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55690
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA3090.5548572454659724No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC1780.3196265038606572No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC1630.29269168611959057No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT1260.2262524690249596No Hit
GGTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAA1150.2065002693481774No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAAT1130.2029089603160352No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT1030.1849524151553241No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA880.1580175974142575No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT720.1292871251571198No Hit
CACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGATATCGAGGCCAAGGG720.1292871251571198No Hit
CGTCGATATAGTCAGAGGTGACAAGATTGGTCTTGTCTTTAGCCATTCCA710.12749147064104865No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT700.12569581612497754No Hit
CGTCGATATAGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC690.12390016160890645No Hit
CGTCGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAACAGGGCTGGA670.12030885257676423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG201.729222E-595.000013
CGTCAGT409.094947E-1295.000011
GTGTCGT950.095.000012
TATATCA201.729222E-595.000017
GCGATAT201.729222E-595.000013
GTCGTCT950.095.000014
GCGCGTC201.729222E-595.000015
GGCGATA201.729222E-595.000012
TACACGC201.729222E-595.000014
CGCTACA201.729222E-595.000011
GCTACAC201.729222E-595.000012
ACGAGTC201.729222E-595.000013
CGGCGAT201.729222E-595.000011
TATCATA950.095.000015
TATCAGA201.729222E-595.000019
GCGTCGA201.729222E-595.000019
GCGTCAC201.729222E-595.000017
CGTCGGT201.729222E-595.000011
CATACGA950.095.000018
CGCGCGT201.729222E-595.000014
Produced by FastQC (version 0.11.5)