FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC316CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC316CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80849
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC5680.7025442491558337No Hit
CGACATACTTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAA3340.4131158084824797No Hit
CGACATACTCACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAA3290.40693144009202337No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA2800.34632462986555185No Hit
CACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGATATCGAGGCCAAGGG2260.27953345124862394No Hit
GGTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAA2230.2758228302143502No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAAT2160.2671647144677114No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT2140.2646909671115289No Hit
CGACATACTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATT2130.2634540934334376No Hit
CGACATACTCACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGG2050.25355910400870757No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC1840.2275847567687912No Hit
CGACATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTC1570.19418916746032727No Hit
CGACATACTCACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTG1020.12616111516530817No Hit
CGACATACTCACGCGTGATCAGCAAAAGCAGGAGTTTAAAATGAATCCAA1010.12492424148721692No Hit
CGACATACTTACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTG990.12245049413103441No Hit
ACGCGTGATCAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCG970.11997674677485187No Hit
CGACATACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTT950.11750299941866936No Hit
CGACATACTCACGGAAACCACTGACTGTCCAGCAGCAAAGTTGGTGTTGC940.11626612574057811No Hit
GTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAAG890.11008175735012184No Hit
CCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAAGGAATGG840.10389738895966556No Hit
CGACATACTTACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATT830.1026605152815743No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT810.10018676792539179No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA201.733991E-595.000018
CGTATAG500.095.000011
GAGTACG254.581616E-795.000016
GAGTACA409.094947E-1295.000014
CGACATT254.581616E-795.000011
CTGACGT450.095.000013
GCGCACT500.095.000017
AGAGTAC409.094947E-1295.000013
ACAGCTA254.581616E-795.000015
GTGTATA900.095.000012
GTCGTCT1100.095.000014
ACGCTGT450.095.000017
CGTGTAT900.095.000011
CGTCGTA500.095.000011
CAGCTAC254.581616E-795.000016
CGAGATG900.095.000011
CGATGTG201.733991E-595.000016
CGAGAGT409.094947E-1295.000011
GCTGTCG500.095.000019
AGCTACT254.581616E-795.000017
Produced by FastQC (version 0.11.5)