FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC313CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC313CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87035
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATAGTCGTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAA4100.4710748549434136No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA2420.27804906072269775No Hit
CGATAGTCGTACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGG2250.2585166886884587No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC2220.2550697995059459No Hit
CGATAGTCGTACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGC1870.2148560923766301No Hit
CGATAGTCGTACTCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCA1570.18038720055150226No Hit
CGATAGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCC1510.1734934221864767No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT1320.15166312403056242No Hit
CGATAGTCGTACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTG1260.14476934566553687No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA1130.12983282587464814No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC1120.12868386281381053No Hit
CGATAGTCGTACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTT1090.12523697363129777No Hit
GGTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAA1020.1171942322054346No Hit
CGATAGTCGTACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGG930.10685356465789625No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT920.10570460159705865No Hit
CGATAGTCGTACTCAGTGATAGAGGCCAAGGGTGTTGCCTCTTCCTTTTA900.10340667547538347No Hit
CGATAGTCGTACTCAGTGCTAGCCAGCACCATTCTGTTTTCATGCCTGAT890.10225771241454587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACT254.584599E-795.000017
AGATCGT254.584599E-795.000014
GTACTTA254.584599E-795.000019
CGCACTC1000.095.000018
GCTACGT254.584599E-795.000016
AATAGTC254.584599E-795.000012
ATACGTG254.584599E-795.000019
GTGACGT500.095.000014
CAGAGTA500.095.000018
AGCCGTA301.218541E-895.05
TCGTTCT156.5972033E-495.07
AGTCATA156.5972033E-495.05
CGTCTCG1100.095.06
TAGCCGT301.218541E-895.04
ACAGCTA156.5972033E-495.05
GTGTATA650.095.02
ACGCTGT450.095.07
GTATCGA156.5972033E-495.05
TACTCGT156.5972033E-495.04
CAGCTAC156.5972033E-495.06
Produced by FastQC (version 0.11.5)