FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC310CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC310CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15535
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA2091.345349211457998No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA1631.0492436433859027No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC1340.8625683939491471No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC1080.6952043772127454No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT900.5793369810106211No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAAAAGCAGGTAGATATTTAAAG710.4570325072417123No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT650.41841004184100417No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT540.3476021886063727No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT480.30897972320566464No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGTAGATATT400.2574831026713872No Hit
CGCTATACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAA330.21242355970389443No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAAT300.1931123270035404No Hit
CGAGACGTAGATGACGAGTATCAGTAGAAACAAGGTAGTTTTTTACTCTA300.1931123270035404No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGGTGACAAA220.14161570646926297No Hit
CGTATAGACGACTAGTACGTGATCAGTAGAAACAAGGTAGTTTTTTACTC220.14161570646926297No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGTAGTTTT210.13517862890247828No Hit
CGTCACGTCGTGTGCGTGCTACGCGTGATCAGTAGAAACAAGGGTGTTTT200.1287415513356936No Hit
CGCTATACGAAGTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTG180.11586739620212425No Hit
CGTATAGACGACTAGTACGTGATCAGCAAAAGCAGGTAGATATTTAAAGA180.11586739620212425No Hit
CGCTATACGACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAA180.11586739620212425No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAAAAGCAGGGTGACAAAAACAT170.10943031863533956No Hit
CGTGTATATACTCTAGCGAGTGATCAGTAGAAACAAGGTAGTTTTTTACT160.10299324106855488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGT353.0195224E-1095.02
CGTCACG254.4077933E-795.08
GTATGAG156.494369E-495.02
GTCACGT254.4077933E-795.09
TATACTC301.1532393E-895.07
CGATAGT156.494369E-495.01
GTGTATA301.1532393E-895.02
AGTAGAC254.4077933E-795.03
TCGCGCG254.4077933E-795.03
TCGCGAG254.4077933E-795.08
GCGCGTC254.4077933E-795.05
GTCGAGA254.4077933E-795.02
CGCTATA1000.095.01
TGATAGT156.494369E-495.07
GCTATAC1050.095.02
AGTACGA156.494369E-495.07
TCAGTCT301.1532393E-895.03
ACGAGTC254.4077933E-795.03
ATAGTCG156.494369E-495.03
ATAGTAT156.494369E-495.09
Produced by FastQC (version 0.11.5)