FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC309CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC309CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31323
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA2700.8619863997701369No Hit
CGTAGTATCTATAGTACTGCTAACGCGAAGTAGGTACAGATGCAATTGTC1990.6353159020528046No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGGTGTTT1880.600197937617725No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA1750.5586948887399036No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC1630.5203843820834531No Hit
CGTAGTATCTATAGTACTGCTAAGCCCATACACACAAGCAGGCAGGCAGG1380.4405708265491811No Hit
CGTAGTATCTATAGTACTGAAAGCGAACTTCAGTGTAATCTTCAACCGAT1300.4150304887782141No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT1110.3543721865721674No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGGTATTT950.3032915110302334No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT930.2969064265874916No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT910.2905213421447499No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT880.28094371548063723No Hit
CGTAGTATCTATAGTACTGCTACCCCTCTTAGTTTGCATAGTTTCCCGTT740.23624812438144496No Hit
CGTAGTATCTATAGTACTGCTACCGCGAAGTAGGTACAGATGCAATTGTC730.23305558216007408No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC670.2139003288318488No Hit
CGTAGTATCTATAGTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATC660.21070778661047793No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAAT630.2011301599463653No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGGGGTTT590.18835999106088178No Hit
CGTAGTATCTATAGTACTGCTACGCGTGATCAGTAGAAACAAGGTCGTTT510.16281965328991474No Hit
ACGCGTGATCAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGC470.15004948440443125No Hit
CGTAGTATCTATAGTACTGCTAACGAGAAAGCTCTTATCTCTTGTTCTAC470.15004948440443125No Hit
CGTAGTATCTATAGTACTGCTAAAACGAGAAAGCTCTTATCTCTTGTTCT420.13408677329757684No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT410.13089423107620599No Hit
CGTAGTATCTATAGTACTGCTAAATCCATGTTGTTCGGGTCCCCATTCCC390.12450914663346424No Hit
CGTAGCAGTACTATAGATACTACGAGATCGGAAGAGCGTCGTGTAGGGAA380.12131660441209335Illumina Single End PCR Primer 1 (100% over 26bp)
CGTAGTATCTATAGTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATT380.12131660441209335No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTATTTT360.1149315199693516No Hit
CGTAGTATCTATAGTACTGCTACTCCCATTCGCTTCTGGTAGGCCTGCAA360.1149315199693516No Hit
CGTAGTATCTATAGTACTGCTAAGGCGAAGTAGGTACAGATGCAATTGTC360.1149315199693516No Hit
CGTAGTATCTATAGTACTGCTACGCCCATACACACAAGCAGGCAGGCAGG350.11173897774798072No Hit
CGTAGTATCTATAGTACTGCTATGGGGCTACCCCTCTTAGTTTGCATAGT350.11173897774798072No Hit
CGTAGTATCTATAGTACTGCTAGGGGGGGGAAAATGGACGAAGGACAAGG350.11173897774798072No Hit
CGTAGTATCTATAGTACTGCTAGAAACGAGAAAGCTCTTATCTCTTGTTC320.10216135108386808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG201.7173414E-595.000011
GCGTCGA201.7173414E-595.000019
CGTCGTA409.094947E-1295.000011
CGAGATG450.095.000011
ACGCGTC201.7173414E-595.000017
CGCGTCG201.7173414E-595.000018
GAGATGA450.095.000012
GTGTCGT353.1650416E-1095.02
GTGTCGC156.557419E-495.02
AGTAGAC156.557419E-495.03
AGCTACG156.557419E-495.05
GATGTGT156.557419E-495.07
TCGCGAG156.557419E-495.08
GAGCTAC156.557419E-495.04
GAGTCGC156.557419E-495.05
ACTCACG301.19307515E-895.07
TCACGTC500.095.03
ACGAGTC156.557419E-495.03
AGACGTA254.5157867E-795.03
TCTCGAC301.19307515E-895.08
Produced by FastQC (version 0.11.5)