FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC307CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC307CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77368
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATACAGAGTAGTACGCGTGATCAGTAGAAACAAGGGTGTTTTTTAT4230.5467376693206494No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA3920.5066694240512873No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC1840.2378244235342777No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT1520.19646365422396855No Hit
CGACATACAGAGTAGTACGCGTGATCAGTAGAAACAAGGGGGTTTTTTAT1510.1951711301830214No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT1270.1641505532002895No Hit
CGACATACAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGAAAT1220.1576879329955537No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT1160.14993278874987076No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA1110.14347016854513495No Hit
CGACATACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACA1060.13700754834039913No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAAT990.12795988005376902No Hit
ACGCGTGATCAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGC780.10081687519387861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGC409.094947E-1295.000018
AGATCGT254.5797606E-795.000014
GTCTACT409.094947E-1295.000016
GAGACGT201.7335196E-595.000012
CGCTACA409.094947E-1295.000011
ACGTCAC201.7335196E-595.000018
GCTACAC409.094947E-1295.000012
ATAGTCT201.7335196E-595.000019
ATAGTAT201.7335196E-595.000019
CGACTGC500.095.000019
CGACTAT201.7335196E-595.000011
CGATAGT201.7335196E-595.000011
GACTATA201.7335196E-595.000012
TACGTCA201.7335196E-595.000017
TATCATA1400.095.05
GCGCGTC450.095.05
GTCTATC1450.095.02
GTCTAGA156.59441E-495.04
GCTGACG156.59441E-495.02
TCGTGTG550.095.08
Produced by FastQC (version 0.11.5)