FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC300CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC300CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67715
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTACACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAA8401.2404932437421545No Hit
CGCTACACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTC5310.7841689433655763No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA5260.7767850550099683No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC4640.6852248394004282No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC3310.48881340914125376No Hit
CGCTACACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTT2610.3854389721627409No Hit
GGTGTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAA1700.25105220409067414No Hit
CGTGTATATACTCTAGCGAGTGATCAGCAAAAGCAGGTAGATATTTAAAG1510.22299342833936353No Hit
CGCTACACGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGT1430.2111792069703906No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA1300.19198109724580964No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT1300.19198109724580964No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT1240.18312043121907995No Hit
CACGATTTGTTTCCCAACAAGAGTGGCTGTTTCGATATCGAGGCCAAGGG1240.18312043121907995No Hit
CGCTACACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCAT1150.16982943217898544No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGCAAAAGCAGGTAGATATT1150.16982943217898544No Hit
CGCTACACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTA1060.15653843313889093No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT1010.14915454478328288No Hit
CGCTACACGCGTGATCAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCA990.14620098944103965No Hit
CGCTACACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGA940.13881710108543158No Hit
CGCTACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAA920.13586354574318835No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT910.13438676807206673No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAAT840.12404932437421545No Hit
CGCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCC710.1048512146496345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACT800.095.000017
ACAGCTA201.7319479E-595.000015
CAGCTAC201.7319479E-595.000016
CGAGATG1900.095.000011
CGCACTC800.095.000018
AGCTACT201.7319479E-595.000017
GCACTCG800.095.000019
ACGACGT201.7319479E-595.000017
CGACGTA254.5735214E-795.08
CGAGTAG850.095.01
AGATACG254.5735214E-795.07
CGTGAGC600.095.01
GAGTACG301.2143573E-895.06
CACGCGA254.5735214E-795.06
GTCTATA450.095.09
CGCGAGT353.237801E-1095.09
CTGACGT156.590822E-495.03
AGAGTAC500.095.03
ACGATAC156.590822E-495.05
GAGACGT353.237801E-1095.02
Produced by FastQC (version 0.11.5)