FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC295CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC295CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14402
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT1170.8123871684488266No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT1030.7151784474378559No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA970.6735175670045828No Hit
ACGCGTGATCAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAAT820.5693653659213999No Hit
CGATACGATACACGCGTATGTACGGCGCCATCAAGGGAGCAATTTTACAA560.38883488404388283No Hit
ACGCGTGATCAGTAGAAACAAGGCCGTTTTTAAACAATTCGACACTAATT450.31245660324954866No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA340.23607832245521454No Hit
CGATACGATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTA340.23607832245521454No Hit
ACGCGTGATCAGTAGAAACAAGGTATTTTTTCATGAAGGACAAGTTAAAT310.215247882238578No Hit
CGATACGATACACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTA270.1874739619497292No Hit
CGATACGATACACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCT270.1874739619497292No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT250.1735870018053048No Hit
ACGCGTGATCAGTAGAAACAAGGCGTTTTTTGAACAAATTACTTGTCAAT220.15275656158866824No Hit
CGATACGATTCACACTCTGGATTTCCCAGGATCCAGCCAGCAATGTTACA210.14581308151645603No Hit
CGATACGATACACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATT210.14581308151645603No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC200.13886960144424387No Hit
ACGCGTGATCAGCAAAAGCAGGCCAAATATATTCAATATGGAGAGAATAA200.13886960144424387No Hit
CGATACGATACACGCGTATGTACGCCGCCATCAAGGGAGCAATTTTACAA170.11803916122760727No Hit
CGATACGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA150.10415220108318289No Hit
ACGCGTGATCAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAA150.10415220108318289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG201.6857548E-595.000019
GTGTCGT201.6857548E-595.000012
ACGCTGT201.6857548E-595.000017
GTCGTCT201.6857548E-595.000014
GCTGTCG201.6857548E-595.000019
TCTCGAC201.6857548E-595.000018
GTCTCGA201.6857548E-595.000017
TCGTCTC201.6857548E-595.000015
TAGACGT201.6857548E-595.000019
CGTCTCG201.6857548E-595.000016
CGTCACG156.484859E-495.01
GTCGTAC156.484859E-495.02
AGACGAT156.484859E-495.06
TATCATA301.1472366E-895.05
GACGATC156.484859E-495.07
CGAGTAG156.484859E-495.01
GTCTATC301.1472366E-895.02
CGTCGTA156.484859E-495.01
ATCATAC301.1472366E-895.06
TCGTACG156.484859E-495.03
Produced by FastQC (version 0.11.5)