FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC294CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC294CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21733
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT2351.081304927989693No Hit
ACGCGTGATCAGTAGAAACAAGGCCGTTTTTAAACAATTCGACACTAATT1490.6855933373211246No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA1250.5751621957391985No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC710.32669212717986473No Hit
ACGCGTGATCAGTAGAAACAAGGCGTTTTTTGAACAAATTACTTGTCAAT670.3082869369162104No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA530.24386877099342014No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC520.23926747342750657No Hit
ACGCGTGATCAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAAT500.23006487829567937No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCT450.2070583904661114No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT410.18865320020245707No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC350.16104541480697557No Hit
GACAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGA290.13343762941149404No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT270.12423503427966685No Hit
ACGCGTGATCAGCAAAAGCAGGCGTTTAAAATGAATCCAAACCAAAAGAT250.11503243914783969No Hit
ACGCGTGATCAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAA230.10582984401601252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATC450.095.000012
ATCATAC450.095.000016
CATACGA450.095.000018
TCATACG450.095.000017
CTACACG156.53001E-495.03
GTATGTA254.468675E-795.02
GTAGACG254.468675E-795.04
CGATAGT254.468675E-795.01
AGTAGAC254.468675E-795.03
GTCGTCT254.468675E-795.04
CGTAGTA156.53001E-495.01
CGTAGAG156.53001E-495.01
CGCTACA156.53001E-495.01
GCTACAC156.53001E-495.02
ATAGTCG254.468675E-795.03
ATACGTA156.53001E-495.06
ACATACA156.53001E-495.03
ATACGAC600.095.09
CGTACTC254.468675E-795.08
TCGTCTC254.468675E-795.05
Produced by FastQC (version 0.11.5)