FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC293CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC293CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATCCTCTCGTCATTGCAGCAAATATCATTGGGATCTTGCACCTGATA50.4646840148698885No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA50.4646840148698885No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC40.37174721189591076No Hit
TCATTGCAGCAAATATCATTGGGATCTTGCACCTGATATTGTGGATTACT30.2788104089219331No Hit
CGGCTTTGAGGGGGCCTGACACGACGTATGTCGCGCGCGAGATCGGAAGA30.2788104089219331No Hit
AGTGATCCTCTCGTCATTGCAGCAAATATCATTGGGATCTTGCACCTGAT30.2788104089219331No Hit
CGGCTTTGAGGGGGCCGGACACGACGTATGTCGCGCGCGAGATCGGAAGA30.2788104089219331No Hit
ACGCGTGATCAGTAGAAACAAGGTGTTTTTTATCATTAAATAAGCTGAAA30.2788104089219331No Hit
ATGCAGCGATTCAAGTGATCCTCTCGTCATTGCAGCAAATATCATTGGGA30.2788104089219331No Hit
TCTTTAGCCATTCCATGAGAGCCTCAAGATCTGTGTTCTTTCCTGCAAAG20.18587360594795538No Hit
GGGTTTTCGGCTGAATCAAGAAAATTGCTTCTCATTGTTCAGGCACTTAG20.18587360594795538No Hit
TGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTGTTTCCCAA20.18587360594795538No Hit
AAGCGAATGGGAGTGCAGATGCAGCGATTCAAGTGATCCTCTCGTCATTG20.18587360594795538No Hit
CGTCTATCATACGACTGTCTACACACGCGTGATCAGTAGAAACAAGGTAG20.18587360594795538No Hit
GTCTTTTTTTCAAATGTATTTATCGTCGCTTTAAATACGGTTTGAAAAGA20.18587360594795538No Hit
AATACGGTTTGAAAAGAGGGCCTTCTACGGAAGGAGTGCCTGAGTCCATG20.18587360594795538No Hit
CAGCAAATATCATTGGGATCTTGCACCTGATATTGTGGATTACTGATCGT20.18587360594795538No Hit
AAGATTGGTCTTGTCTTTAGCCATTCCATGAGAGCCTCAAGATCTGTGTT20.18587360594795538No Hit
CGTCGCGCGTCACGTATGACTGAAACAAGGTACTTTTTTGGACAGTATGG20.18587360594795538No Hit
AGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCC20.18587360594795538No Hit
CAATAAAGCAAGATATCGTAGGAATAAATGAGTGGTCAGGATATAGCGGG20.18587360594795538No Hit
AGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGGGTGGAGT20.18587360594795538No Hit
TAAGTGTCTCGCTGGATCCCTCTTTCAAGATCCATTCCACGATTTGTTTC20.18587360594795538No Hit
CAGGCCTACCAGAAGCGAATGGGAGTGCAGATGCAGCGATTCAAGTGATC20.18587360594795538No Hit
TTTACCCAAGTGTTGTTTTCATAAGTAATGACGCTTTGATTGCATGTTTC20.18587360594795538No Hit
TGGATTAGTCGTCCATCATAATCACTGAGTTTGAGTTCAGTGCACATTTG20.18587360594795538No Hit
TGATCGTCTTTTTTTCAAATGTATTTATCGTCGCTTTAAATACGGTTTGA20.18587360594795538No Hit
GATGAAGGCACATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCAT20.18587360594795538No Hit
TGTTTCCCAACAAGAGTGGCTGTTTCGATATCGAGGCCAAGGGTGTTGCC20.18587360594795538No Hit
CCTCAAGATCTGTGTTCTTTCCTGCAAAGACACTTTCCAGTCTCTGCGCG20.18587360594795538No Hit
TATTTATCGTCGCTTTAAATACGGTTTGAAAAGAGGGCCTTCTACGGAAG20.18587360594795538No Hit
GTGAATCAGCAATCTGTTCACAAGTGGCACACACTAGACCAAAAGCAGCT20.18587360594795538No Hit
CTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTC20.18587360594795538No Hit
AGAATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGC20.18587360594795538No Hit
ATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG20.18587360594795538No Hit
AATGGGAGTGCAGATGCAGCGATTCAAGTGATCCTCTCGTCATTGCAGCA20.18587360594795538No Hit
ATACGGTTTGAAAAGAGGGCCTTCTACGGAAGGAGTGCCTGAGTCCATGA20.18587360594795538No Hit
CGGTGAGCGTGAACACAAATCCTAAAATTCCCTTAGTCAGAGGTGACAAG20.18587360594795538No Hit
CAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCT20.18587360594795538No Hit
CCTAATTATTACTATGAGGAATGCTCCAGTTATCCCTGCACACACATGTG20.18587360594795538No Hit
ACGCGTGATCAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCT20.18587360594795538No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)