FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC292CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC292CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52413
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA3730.7116555053135672No Hit
ACGCGTGATCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACAC3570.6811287276057467No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA2930.5590216167744643No Hit
ACGCGTGATCAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACC1870.356781714460153No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT1660.3167153187186385No Hit
ACGCGTGATCAGTAGAAACAAGGCAGTTTTTTACTCTAGCTCTATGTTGA1500.2861885410108179No Hit
CGAGATGAGTCTATATACGCGTGATCAGTAGAAACAAGGGTGTTTTTTAT1250.23849045084234827No Hit
ACGCGTGATCAGCAAAAGCAGGTGACAAAAACATAATGGACTCCAACACC1220.23276668002213194No Hit
ACGCGTGATCAGTAGAAACAAGGCGTTTTTTGAACAAATTACTTGTCAAT1200.22895083280865436No Hit
ACGCGTGATCAGTAGAAACAAGGCCGTTTTTAAACAATTCGACACTAATT990.18888443706713984No Hit
CGAGATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGT980.18697651346040103No Hit
CGAGATGAGTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCAT910.17362104821322955No Hit
ACGCGTGATCAGCAAAAGCAGGCAGATATTTAAAGATGAGTCTTCTAACC840.16026558296605803No Hit
CGAGATGAGTCTATATACGCGTGATCAGTAGAAACAAGGGGGTTTTTTAT820.15644973575258048No Hit
ACGCGTGATCAGCAAAAGCAGGCGACAAAAACATAATGGACTCCAACACC780.14881804132562532No Hit
ACGCGTGATCAGCAAAAGCAGGTTGACAAAAACATAATGGACTCCAACAC720.1373704996851926No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT650.1240150344380211No Hit
ACGCGTGATCAGCAAAAGCAGGCGCATTGACTGAAGATCCAGATGAAGGC640.12210711083128231No Hit
ACGCGTGATCAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAA630.12019918722454354No Hit
CGAGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGA610.11638334001106597No Hit
GATAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGA570.10875164558411081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGC550.095.02
CACGTCG450.095.04
AGCTACG301.2090823E-895.05
GCGCGTC254.5589877E-795.05
GTACACA156.5824256E-495.06
CGCTATC353.2196112E-1095.01
TAGTCGC156.5824256E-495.04
CAGAGTA353.2196112E-1095.08
GCTACGT301.2090823E-895.06
GTGAGCT301.2090823E-895.02
GAGTTAT156.5824256E-495.07
GCTATCA353.2196112E-1095.02
ACTCACG156.5824256E-495.07
TCACGTC450.095.03
AGACGTA156.5824256E-495.03
AGAGTAC156.5824256E-495.03
GAGTAGA353.2196112E-1095.02
GACGTAG156.5824256E-495.04
AGACGAC600.095.06
CGTATAG600.095.01
Produced by FastQC (version 0.11.5)