FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC263CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC263CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17235
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA530.3075137800986365No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA250.1450536698578474No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT220.12764722947490573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGT156.5066846E-495.02
TCTAGAT156.5066846E-495.05
GTCGTCT156.5066846E-495.04
ACTCACG156.5066846E-495.07
ACATACT156.5066846E-495.03
CGTACTC254.4287845E-795.08
GTCTAGA156.5066846E-495.04
TCGTACT254.4287845E-795.07
CTAGATA156.5066846E-495.06
CTCACGC156.5066846E-495.08
TACTCAC156.5066846E-495.06
CGCGTCT156.5066846E-495.01
TAGATAC156.5066846E-495.07
TGTCGTC156.5066846E-495.03
CGTCTCG156.5066846E-495.06
CGTCTAG156.5066846E-495.03
CGATAAC201.6952361E-594.999998
GACATAC201.6952361E-594.999992
CGTGTCG201.6952361E-594.999991
ACGATAA201.6952361E-594.999997
Produced by FastQC (version 0.11.5)