FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC262CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC262CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13644
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAAT1330.9747874523600116No Hit
ACGCGTGATCAGTAGAAACAAGGTATTTTTTCATGAAGGACAAGTTAAAT1200.8795074758135445No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC730.5350337144532394No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT300.21987686895338612No Hit
CACCATGGACACAGTGGTGGAGGCCATGGTGTCTAGGGCCCGGATTGATG220.16124303723248312No Hit
ACGCGTGATCAGCAAAAGCAGGTAAACCATTTGAATGGATGTCAATCCGA220.16124303723248312No Hit
ACGCGTGATCAGTAGAAACAAGGCGTTTTTTGAACAAATTACTTGTCAAT200.1465845793022574No Hit
ACGCGTGATCAGTAGAAACAAGGCCGTTTTTAAACAATTCGACACTAATT170.1245968924069188No Hit
CGACGACAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGT160.11726766344180592No Hit
ACGCGTGATCAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGA140.10260920551158018No Hit
ACGCGTGATCAGCAAAAGCAGGCATTTTTTCATGAAGGACAAGTTAAATT140.10260920551158018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGTA156.4769824E-495.02
GTGTCGC156.4769824E-495.02
TATACTC156.4769824E-495.07
GTGTATA156.4769824E-495.02
GTCGCGT254.3782347E-795.06
CACGTCG156.4769824E-495.04
TATGTAT156.4769824E-495.03
TCGCGTG254.3782347E-795.07
GTACACT156.4769824E-495.08
CTGACGT156.4769824E-495.03
TACACTC156.4769824E-495.09
TAGTCGC254.3782347E-795.04
GCGTGTA254.3782347E-795.09
CGCTAGT254.3782347E-795.01
ACTCACG156.4769824E-495.07
TCACGTC156.4769824E-495.03
CGTATGT156.4769824E-495.01
GCTGACG156.4769824E-495.02
CTAGTCG254.3782347E-795.03
GCGCACT156.4769824E-495.07
Produced by FastQC (version 0.11.5)