FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC258CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC258CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68223
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGGTGTTTTCTGTTAGATGCCAGGAGGT6380.9351684915644284No Hit
ACGCGTGATCAGTAGAAACAAGGGGCATCACTGTCACAGTGGTGAGTGAT4140.6068334725825602No Hit
ACGCGTGATCAGTAGAAACAAGGAGTTGAACTTTGTTTTCTTGATTTGAA3010.4412001817568855No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGCAAAAGCAGGAGCAAGAGAG1500.21986720021107253No Hit
ACGCGTGATCAGTAGAAACAAGGGTGAGAAGATGTAGGAGCTGTCCACCC1210.1773595415035985No Hit
ACGCGTGATCAGCAAAAGCAGGGACTCTGTCTTAGTCACTACTTCATCCC1140.1670990721604151No Hit
ACGCGTGATCAGCAAAAGCAGGAGCAAGAGAGACTGAGTGTGAGACACCA1060.15537282148249126No Hit
ACGCGTGATCAGTAGAAACAAGGGCCCTTCATGTTTCTCACACTCCCTTG1050.15390704014775075No Hit
ACGCGTGATCAGTAGAAACAAGGTGATCTTCCAGGAGCTAATGGTAGTGC1030.15097547747826978No Hit
CGTAGAGTATCTGAGTAACGTGTGAAAAACAGATTTTAAGTTGTAAGTGC970.1421807894698269No Hit
CGTAGAGTATCTGAGTATACGCGTGATCAGTAGAAACAAGGAGTTGAACT870.12752297612242205No Hit
ACGCGTGATCAGCAAAAGCAGGAATCACTCTAGAATTATGCCTTTGACAT810.11872828811397916No Hit
CGTAGAGTATCTGAGTATACGCCGGCGCCAAACCTTATGTGAAATTGTAA780.11433094410975769No Hit
ACGCGTGATCAGTAGAAACAAGGAGATCTTCAGCAAATCCTCACTTTTTA770.11286516277501722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGA201.7320399E-595.000015
TAGCGTA254.5739034E-795.000013
GCGCACT254.5739034E-795.000017
GTAGCGT254.5739034E-795.000012
GTAGAGC201.7320399E-595.000012
CGCTATA201.7320399E-595.000011
CGTCGTA409.094947E-1295.000011
GCTACGT201.7320399E-595.000016
AGCTACG201.7320399E-595.000015
TGTCGCG201.7320399E-595.000013
TATCATA550.095.05
CGTATAG156.591032E-495.01
CATACGA450.095.08
TGTAGAG156.591032E-495.01
CGAGAGT301.2145392E-895.01
GTACGCT353.237801E-1095.05
CGTAGAG27150.093.250461
GTAGAGT27400.091.70622
TAGAGTA27750.089.522533
AGAGTAT28100.087.562284
Produced by FastQC (version 0.11.5)