FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC257CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC257CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20322
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGCAAAAGCAGGTTGATGTGCTAAATGTCAATTATTTCAA470.23127644916838894No Hit
ACGCGTGATCAGCAAAAGCAGGCTGATGTGCTAAATGTCAATTATTTCAA350.17222714299773645No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT340.1673063674835154No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA330.16238559196929436No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA320.15746481645507332No Hit
CGCATAGTCGTACGTATACATCCGGCCATCTGTTCCATAGCCTTTGCCGT240.11809861234130499No Hit
ACGCGTGATCAGTAGAAACAAGGCGATCTTCCAGGAGCTAATGGTAGTGC210.10333628579864186No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGA201.7027072E-595.000012
GTCTATC254.4581247E-795.000012
CGTGTCG254.4581247E-795.000011
CGTCGAG201.7027072E-595.000011
CATACGA201.7027072E-595.000018
TCGAGAC201.7027072E-595.000013
CGTCTAT254.4581247E-795.000011
GTAGAGT156.523856E-495.02
GTGTCGT156.523856E-495.02
GTCGTCT156.523856E-495.04
CGTAGAG156.523856E-495.01
TAGTCGA156.523856E-495.05
GAGTCGC156.523856E-495.05
GAGTATA301.171793E-895.02
TCTCGAC156.523856E-495.08
GTCTCGA156.523856E-495.07
TCGTCTC156.523856E-495.05
GACGAGA156.523856E-495.07
CGAGTCG156.523856E-495.04
CGTGTAT156.523856E-495.01
Produced by FastQC (version 0.11.5)