FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC248CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC248CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60735
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA4360.7178727257759118No Hit
ACGCGTGATCAGTAGAAACAAGGCGATCTTCCAGGAGCTAATGGTAGTGC2470.40668477813451887No Hit
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT2400.39515929859224497No Hit
ACGCGTGATCAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAA1900.3128344447188606No Hit
ACGCGTGATCAGTAGAAACAAGGTGATCTTCCAGGAGCTAATGGTAGTGC1800.2963694739441837No Hit
ACGCGTGATCAGTAGAAACAAGGCCGTTTTTAAACAATTCGACACTAATT1430.2354490820778793No Hit
ACGCGTGATCAGTAGAAACAAGGCGTTTTTTGAACAAATTACTTGTCAAT1380.22721659669054087No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA1270.2091051288383963No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGGTGTTTT970.15971021651436568No Hit
CGATACAGCTACTACTACTCTAATTCGCTTCTGGTAGGCCTGCAAATTTT780.12842677204247963No Hit
ACGCGTGATCAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAAT770.12678027496501193No Hit
ACGCGTGATCAGTAGAAACAAGGCACTTTTTTGGACAGTATGGATAGCAA720.1185477895776735No Hit
CGATACAGCTACTACTACTCTACGCGTGATCAGTAGAAACAAGGTCGTTT690.11360829834527043No Hit
CGATACAGCTACTACTACTCTAACGCGAAGTAGGTACAGATGCAATTGTC640.10537581295793201No Hit
CGATACAGCTACTACTACTCTACGTCCCCTTGCCCAATTAGCACATTAGC610.10043632172552894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTATA201.7304954E-595.000017
TACAGTA201.7304954E-595.000014
TGATACA156.587505E-495.01
GATACAA156.587505E-495.02
AGATCGT156.587505E-495.04
CGAGTCG700.095.04
CGACGAG700.095.01
GCGCGTC301.2123564E-895.05
GAGTATA550.095.02
GAGTACG156.587505E-495.06
GAGTACA301.2123564E-895.04
GCTATCA156.587505E-495.02
CGTCTCG500.095.06
GTCTAGA156.587505E-495.04
ATACTCG254.5677734E-795.06
AGAGTAC301.2123564E-895.03
TCGTGTG550.095.08
GAGACGT156.587505E-495.02
CGCTATC156.587505E-495.01
CGCTACA156.587505E-495.01
Produced by FastQC (version 0.11.5)