FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC236CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC236CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12203
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGTGATCAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATT1321.0817012210112267No Hit
ACGCGTGATCAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGA990.81127591575842No Hit
ACGCGTGATCAGTAGAAACAAGGCCGTTTTTAAACAATTCGACACTAATT750.6146029664836515No Hit
ACGCGTGATCAGCAAAAGCAGGTTGATGTGCTAAATGTCAATTATTTCAA630.5162664918462673No Hit
ACGCGTGATCAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAA520.4261247234286651No Hit
ACGCGTGATCAGCAAAAGCAGGCTGATGTGCTAAATGTCAATTATTTCAA460.37695648610997295No Hit
ACGCGTGATCAGTAGAAACAAGGTGATCTTCCAGGAGCTAATGGTAGTGC320.2622305990330247No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTAGTTTT310.2540358928132426No Hit
ACGCGTGATCAGTAGAAACAAGGCAGTTTTTTACTCTAGCTCTATGTTGA300.24584118659346063No Hit
CGCGTATACTCACACAAAATCCTCAGAAGGTAGAGGATGAGGAAACACTT250.2048676554945505No Hit
ACGCGTGATCAGTAGAAACAAGGCGATCTTCCAGGAGCTAATGGTAGTGC240.1966729492747685No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAAAGAACGTACGTTTCGACC220.18028353683520446No Hit
CGCGTATACTCTTTCTCACACACATGTCATGCTTTGTTTTCCTTATAGCA200.1638941243956404No Hit
CGTCTATCATACGACTGTCTACGCGTGATCAGTAGAAACAAGGTCGTTTT170.13931000573629435No Hit
CGCGTATACTGTTCACCACTGTCCAGGCCATGATTCGGTCTCTTCCTTCA140.1147258870769483No Hit
CGCGAGTGCGGGACTGCGACATTAGATCTCTCAGTTCTTTTATTCTCTCC140.1147258870769483No Hit
ACGCGTGATCAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAA130.10653118085716627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATG201.6752949E-595.000017
CACGTCG201.6752949E-595.000014
ACGTAGA201.6752949E-595.000015
TCGTGTG201.6752949E-595.000018
TCACGTC201.6752949E-595.000013
AGACGTA201.6752949E-595.000013
TCGTACG201.6752949E-595.000013
TACTCGC900.095.000017
TAGATGA201.6752949E-595.000018
GAGACGT201.6752949E-595.000012
GTATGTA156.4607314E-495.02
GTAGAGT156.4607314E-495.02
ACTCGCA700.095.08
CGATACA156.4607314E-495.01
GTGTATA156.4607314E-495.02
AGCTACG156.4607314E-495.05
TATGTAT156.4607314E-495.03
GAGCTAC156.4607314E-495.04
CGTAGAG156.4607314E-495.01
GCTACGT156.4607314E-495.06
Produced by FastQC (version 0.11.5)