FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC219CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC219CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233722
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTCGAGTGCGCGACACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG34551.4782519403393777Illumina Single End PCR Primer 1 (100% over 30bp)
CGTGTCCCGACTCCTTTGACTGCGGCGCCTGCTGCTCCAGATCTTCTAGG7130.30506328030737373No Hit
CGTGTCTCTCACTCTCCTCTTTCTTTGAAAATGAGTTGTGACCTCTACTT3940.16857634283464973No Hit
CGTCCCTCTATTATCTCAAATCTGTGCTTTAAAAGTGCATTTGGGTCCTC3150.13477550252008796No Hit
CGGCTTTGAGGGGGCCTGACGGGATGATAGAGAGAACGTACGTTTCGACC3080.1317804913529749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCG156.611273E-495.01
GTCGCGT156.611273E-495.06
ATCTATA301.22763595E-895.07
GTCGATA156.611273E-495.02
GACGTAC156.611273E-495.05
TGACGTA156.611273E-495.04
TATCTAT301.22763595E-895.06
CTAGTCG156.611273E-495.03
CTACGTC156.611273E-495.07
CGCTATC1200.095.01
CGCTAGT156.611273E-495.01
GTGTCGT1350.095.02
TCTCGCA254.6090645E-795.06
CTCGCAT254.6090645E-795.07
TCGCGTG156.611273E-495.07
TAGTCGC156.611273E-495.04
TCGCGAC156.611273E-495.05
GTCGCCC201.7409113E-594.999994
CGCTCAT201.7409113E-594.999991
GTCGTAT201.7409113E-594.999992
Produced by FastQC (version 0.11.5)