FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC216CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC216CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99042
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTGTGACTGTTCTCAAGCAGGTTGACAGAGTGTGTCACTGTCACATT2260.2281860220916379No Hit
CGAGTGTGATGATTCTTTGGTTGCATATTCCAGTGTTGTTTTGACTTCCA2200.22212798610690412No Hit
CGAGTGTGATCCTCTTGTTGTTGCCGCAAGTATAATTGGGATTGTGCACT1920.19385715151147997No Hit
CGAGTGTGATAGTATCTCCGGTTATGGTGAAAGAAATTTCAGTGTCCTGG1620.16356697158781125No Hit
CGAGTGTGATCATGGCCAAGAACATTCTAGGGTTTTGATTCTCGTTCCAT1440.14539286363360998No Hit
CGAGTGTGATAGTACCGCGAAGCAAGTGCGGAGGCCATGGTCATTTTAAG1410.1423638456412431No Hit
CGAGTGTGATTCACCCAGGTGCTGTTTTCATATGTGATGATTCTTTGGTT1350.13630580965650937No Hit
CGAGTGTGATAGTATCACTATTAACGCCACAAAAAGAAATGCTGCTCCCA1240.12519941035116416No Hit
CGAGTGTGATAGTATCTCGTAGCTGAAACGAAAACGTTCTAATCTCTTGT1030.10399628440459603No Hit
CGAGTGTGAAAGGTTCTCTTATGACAAAAACATCTCCTTTGGAGCCAATT1000.10096726641222915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTG1700.095.000018
ACTATAC450.095.03
CTCACGC254.5893285E-795.08
GATCGTC500.095.05
CGCGCAC1300.095.06
CGTCTCG254.5893285E-795.06
CGTCTAT156.599919E-495.01
AGGGTGA254.5893285E-795.03
GTCTACT254.5893285E-795.06
GCGCACT1350.095.07
GTGTGAC650.095.04
ACGATGT650.095.05
TGCGATA156.599919E-495.05
GTGTCGC1300.095.02
GTACTCA254.5893285E-795.09
CGAATGT156.599919E-495.01
CATACGA156.599919E-495.08
GCGATAG156.599919E-495.06
TACTCAC254.5893285E-795.06
CGTGTGC1650.095.09
Produced by FastQC (version 0.11.5)