FastQCFastQC Report
Wed 3 Apr 2019
HTKZQN1_l01_n02_BC193CG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTKZQN1_l01_n02_BC193CG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455044
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGTATAGACGTGATACTGAAACGAGAAAGCTCTTATCTCTTGTTCT36770.8080537266725856No Hit
CGTATGTATAGACGTGATACTGAAGAGCTTCCCAGCACTGGGATGCTCTT31660.6957568938388375No Hit
CGTATGTATAGACGTGATACTGTGTCCATGGACACAGTAAACAGAACACA30320.6663091920781288No Hit
CGTATGTATAGACGTGATACTGGCGGGGAGCGCCAGGATGCCACAGAAAT29740.6535631719130458No Hit
CGTATGTATAGACGTGATACTGTGTCCATGGAACAGGAACAGGATACACC21560.47380033579170366No Hit
CGTATGTATAGACGTGATACTGGACCACAACTGCCTGTCTTATCATTAGG18990.4173222809222844No Hit
CGTATGTATAGACGTGATACTGATGCAATTGTCGTTCTAAGTGTCTCGCT15390.33820905231142484No Hit
CGTATGTATAGACGTGATACTGTTTTCTTAGGGTCCTTCCCAGCACTGGG13460.29579557141726953No Hit
CGTATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTG11530.2533820905231143No Hit
CGTATGTATAGACGTGATACTGAAAAGGGAAAGATAGTCAAATCAGTCGA11260.24744859837729977No Hit
CGTATGTATAGACGTGATACTGAAGATCTTGTATGAGGCCTGTCCATCAC10110.22217631701549742No Hit
CGTATGTATAGACGTGATACTGCAAGCCCATACACACAAGCAGGCAGGCA9080.19954114327405703No Hit
CGTATGTATAGACGTGATACTGAAACAAGGCATTTTTTCATGAAGGACAA8440.18547656929879308No Hit
CGTATGTATAGACGTGATACTGAACAAAACTCCCGCTATATCCTGACCAC7660.16833536976644017No Hit
CGTATGTATAGACGTGATACTGAGAAACAAGGCATTTTTTCATGAAGGAC7220.15866597515844622No Hit
CGTATGTATAGACGTGATACTGAGGCCTATTCAACTGGTGCACTTGCCAG7100.15602886753808423No Hit
CGTATGTATAGACGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTAC6890.15141392920245075No Hit
CGTATGTATAGACGTGATACTGTTTACACCACAAAAGGATATGCTGCTCC6820.14987561642390626No Hit
CGTATGTATAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAG6810.14965585745554275No Hit
CGTATGTATAGACGTGATACTGATCCCCTCAGAATGAGTGCTGACCGTGC6700.14723850880354428No Hit
CGTATGTATAGACGTGATACTGTTACATTCTTTTCTAGTACTGTGTCTAC6510.1430630884046378No Hit
CGTATGTATAGACGTGATACTGTTTAAGGTCTCCAACATCTTTGCAGTCA6450.1417445345944568No Hit
CGTATGTATAGACGTGATACTGAATAGCATAACAATAGAAAGCACCATCC6350.1395469449108218No Hit
CGTATGTATAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGC6080.13361345276500736No Hit
CGTATGTATAGACGTGATACTGATTAGCAACCTCCATGGCCTCTGCTGCC6000.13185538101809935No Hit
CGTATGTATAGACGTGATACTGGACGGATCATGATCTCAGAGAACTCTTC5890.12943803236610085No Hit
CGTATGTATAGACGTGATACTGAAGCGAATGGGAGTGCAGATGCAGCGAT5850.12855899649264685No Hit
CGTATGTATAGACGTGATACTGGTGGGGAGCGCCAGGATGCCACAGAAAT5470.12020815569483391No Hit
CGTATGTATAGACGTGATACTGAGCTCAATTGCTCTCTTAGCTCCTCATA5390.11845008394792592No Hit
CGTATGTATAGACGTGATACTGAAATGGGAGGCTGGTGTTTATAGCACCC5380.1182303249795624No Hit
CGTATGTATAGACGTGATACTGCTGAGCTTTGGGTATGAATTTCCTTTTT5350.11757104807447191No Hit
CGTATGTATAGACGTGATACTGGGAGGCTGGTGTTTATAGCACCCTTGGG4890.10746213552975098No Hit
CGTATGTATAGACGTGATTTTGATTCCCAAGTTGAATTGAGTGGCTAATC4880.10724237656138746No Hit
CGTATGTATAGACGTGATACTGATGGACGACTAAAGCAGAAAGCACCATC4750.10438550997266198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCAC201.742693E-595.000018
CACGTCG850.095.000014
CGACTAT201.742693E-595.000011
GTACGCT850.095.000015
CTAGACG156.6153356E-495.08
CTATAGT156.6153356E-495.09
ATAGCCG254.6161404E-795.08
TCTAGAC156.6153356E-495.07
ATGACGT254.6161404E-795.08
CTACACG900.095.03
ACGAGTC353.2923708E-1095.03
TACACGT156.6153356E-495.09
TCGCGCG353.2923708E-1095.03
CGTATGT274150.094.272291
CGTGTCG3150.093.4920651
GTATGTA279150.092.481642
TAGACCT1650.092.1212169
ATCATAC1550.091.9354866
GTATAGA277700.090.569866
TATAGAC277250.090.3742147
Produced by FastQC (version 0.11.5)